A Novel Bioimplant Comprising Ad-BMP9-Transfected BMSCs and GelMA Microspheres Produced from Microfluidic Devices for Bone Tissue Engineering

作者全名："Nie, Li; Liu, Wei; Chen, Jiajun; Zhou, Siqi; Liu, Chang; Li, Wenhui; Ran, Zhiyue; Liu, Yaxian; Hu, Jing; Zhang, Yuxin; Zheng, Liwen; Ji, Ping; Zhang, Hongmei"

作者地址："[Nie, Li; Liu, Wei; Chen, Jiajun; Zhou, Siqi; Liu, Chang; Li, Wenhui; Ran, Zhiyue; Liu, Yaxian; Hu, Jing; Zhang, Yuxin; Zheng, Liwen; Ji, Ping; Zhang, Hongmei] Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China; [Nie, Li; Liu, Wei; Chen, Jiajun; Zhou, Siqi; Liu, Chang; Li, Wenhui; Ran, Zhiyue; Liu, Yaxian; Hu, Jing; Zhang, Yuxin; Zheng, Liwen; Ji, Ping; Zhang, Hongmei] Chongqing Municipal Key, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Nie, Li; Liu, Wei; Chen, Jiajun; Zhou, Siqi; Liu, Chang; Li, Wenhui; Ran, Zhiyue; Liu, Yaxian; Hu, Jing; Zhang, Yuxin; Zheng, Liwen; Ji, Ping; Zhang, Hongmei] Lab Oral Biomed Engn Higher Educ, Chongqing 401147, Peoples R China"

通信作者："Ji, P; Zhang, HM (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China.; Ji, P; Zhang, HM (通讯作者)，Chongqing Municipal Key, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China.; Ji, P; Zhang, HM (通讯作者)，Lab Oral Biomed Engn Higher Educ, Chongqing 401147, Peoples R China."

来源：JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001017676600001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：2023

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Oral and maxillofacial bone defect repair in patients remains challenging in clinical treatment due to the different morphologies of bone defects. An injectable hydrogel of microspheres with sustained bone morphogenetic protein 9 (BMP9) expression for oral and maxillofacial bone defect repair has been developed. This study is bioinspired by the substantial osteogenesis property of recombinant adenoviruses expressing bone morphogenetic protein 9 (Ad-BMP9) and minimally invasive treatment by injection. A novel scaffold encompassing bone mesenchymal stem cells (BMSCs) transfected with Ad-BMP9 was produced and cocultured on a superficial surface of monodisperse photocrosslinked methacrylate gelatin hydrogel microspheres (GelMA/MS, produced with microfluidic technology). The biological tests including live/dead cell staining, phalloidin staining, cell counting kit-8 (CCK-8) assay, alkaline phosphatase (ALP) activity and staining, alizarin red S staining, and quantitative real-time polymerase chain reaction (RT-qPCR), revealed that the hydrogel microspheres exhibited good biocompatibility and remarkably promoted the osteogenic differentiation of BMSCs in vitro. In addition, a small needle was injected the innovative scaffold beneath the nude mice's skin. The micro-CT and histological staining assay results demonstrated that the new implant, with high blood vessel formation markers (CD31-positive cells) expression over four and eight weeks, achieved significant vascularized bone-like tissue formation. Consequently, the injectable hydrogel microspheres, cocultured with BMSC transfected with Ad-BMP9, enhanced vascularized bone regeneration, therefore representing a facile and promising technique for the minimally invasive treatment of oral and maxillofacial bone defects."

基金机构：Natural Science Foundation of Chongqing [cstc2021jcyjmsxmX0560]; New Technology and New Project of Chongqing Medical University Affiliated Stomatological Hospital [QYYL2022X020]

基金资助正文：AcknowledgmentsThe reported work was supported by research grants from the Natural Science Foundation of Chongqing (Grant no. #cstc2021jcyjmsxmX0560 to HZ) and New Technology and New Project of Chongqing Medical University Affiliated Stomatological Hospital (Grant no. QYYL2022X020).