Altered intestinal microbiota in children with bronchiolitis

作者全名："Wu, Xiao-bin; Wang, Jian; Tang, Yuan; Jiang, Jing; Li, Xue-mei"

作者地址："[Wu, Xiao-bin; Wang, Jian; Tang, Yuan; Jiang, Jing; Li, Xue-mei] Chongqing Hlth Ctr Women & Children, Chongqing, Peoples R China; [Wu, Xiao-bin; Wang, Jian; Tang, Yuan; Jiang, Jing; Li, Xue-mei] Chongqing Med Univ, Women & Childrens Hosp, Chongqing, Peoples R China"

通信作者："Jiang, J; Li, XM (通讯作者)，Chongqing Hlth Ctr Women & Children, Chongqing, Peoples R China.; Jiang, J; Li, XM (通讯作者)，Chongqing Med Univ, Women & Childrens Hosp, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001017972400001

JCR分区：Q2

影响因子：4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：bronchiolitis; intestinal microbiota; metabolism; Clostridium; Sphingomonas

摘要："ObjectiveTo investigate the correlation between the alteration of intestinal microbiota and disease in children with bronchiolitis. MethodsFifty seven children diagnosed with bronchiolitis from January 2020 to January 2022 in our pediatric department were included as the case group, and another 36 normal children were included as the control group. Stool and blood were collected from both groups for high-throughput sequencing, untargeted metabolite detection and ELISA. A mouse model of RSV infection was established to validate the results of clinical case detection. ResultsBody weight, passive smoking, and a host of other factors were possible as acute bronchiolitis influencing factors in the onset of acute bronchiolitis. The alpha diversity Shannon, Simpson and Pielou's evenness indices were significantly lower in children with acute bronchiolitis than in healthy children with gated levels of Firmicutes, Bacteroidetes and genus levels of Clostridium and other short chain fatty acid-producing bacteria. The relative abundance of short-chain fatty acid (SCFAs)-producing bacteria decreased and the abundance of genus-level sphingolipid-producing bacteria Sphingomonas increased; the progression of acute bronchiolitis is likely to be associated with the abundance of Clostridium and Sphingomonas and higher fecal amino acid concentrations, including FF-MAS, L-aspartic acid, thioinosinic acid, picolinic acid; supplementation with Clostridium butyricum significantly alleviated RSV infection-induced lung inflammation. ConclusionThe progression of bronchiolitis may be associated with altered intestinal microbiota, decreased SCFAs and elevated sphingolipids metabolism in children. Some fecal bacteria and metabolites may predict the onset of bronchiolitis, and oral administration of Clostridium butyricum may alleviate RSV infection-induced pulmonary inflammation."

基金机构：Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) [2019ZDXM017]; Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0327]; Chongqing Yuzhong District's Fundamental Research and Frontiers Exploration Project [20200146]

基金资助正文："This study was supported by the Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) (no. 2019ZDXM017), Natural Science Foundation of Chongqing (no. cstc2020jcyj-msxmX0327), and Chongqing Yuzhong District's Fundamental Research and Frontiers Exploration Project (no. 20200146)."