Fluorimetric monitoring of vancomycin using an allosteric probe-initiated sensing platform

作者全名："Yuan, Jinshan; Xie, Guoming; Li, Junjie; Xie, Yaxing; Yao, Yuan; Zhang, Jianhong; Hou, Yulei; Chen, Hui"

作者地址："[Yuan, Jinshan; Xie, Yaxing; Zhang, Jianhong; Hou, Yulei; Chen, Hui] Chongqing Med Univ, Clin Labs, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Xie, Guoming; Li, Junjie] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Chinese Minist Educ, Chongqing 400016, Peoples R China; [Yao, Yuan] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Chen, Hui] Chongqing Med Univ, Clin Labs, Affiliated Hosp 1, 1 You Yi Rd, Chongqing 400016, Peoples R China"

通信作者："Chen, H (通讯作者)，Chongqing Med Univ, Clin Labs, Affiliated Hosp 1, 1 You Yi Rd, Chongqing 400016, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001018121700001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：1269

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Vancomycin; Allosteric probe; Hybridization chain reaction; Fluorescence resonance energy transfer; Biosensor

摘要："Vancomycin is the first-line drug for infections of methicillin-resistant Staphylococcus aureus (MRSA) and multi-drug-resistant bacteria. The effective therapeutic concentration range of vancomycin is narrow, so it's essential to implement vancomycin therapeutic drug monitoring. However, conventional detection methods have disadvantages of expensive equipment, complicated operation, or poor reproducibility. Herein, a fluorescent sensing platform initiated by an allosteric probe was constructed for simple and sensitive monitoring of vancomycin at a low cost. The key point of this platform is the well-designed allosteric probe, which comprises an aptamer and a trigger sequence. When vancomycin exists, the combination of vancomycin and the aptamer will lead to a conformational change of the allosteric probe, thus exposing the trigger sequence. The trigger can react with the molecular beacon (MB) to generate fluorescent signals. In addition, the allosteric probe combined with hybridization chain reaction (HCR) was applied to develop an amplified platform, the linear range is from 0.5 & mu;g mL-1 to 50 & mu;g mL-1 with the limit of detection (LOD) of 0.26 & mu;g mL-1. Most importantly, this allosteric probe-initiated sensing platform shows good detection ability in human serum samples, and it also indicates great correlation and accuracy compared with HPLC. The present simple and sensitive allosteric probe-initiated platform has the potential to support the therapeutic drug monitoring of vancomycin, which is of great significance to promote the rational use of antibiotics in clinics."

基金机构：National Natural Science Foundation of China [81972011]; Natural Science Foundation of Chongqing [CSTB2022NSCQ-MSX0101]

基金资助正文：This work was supported by the National Natural Science Foundation of China (No. 81972011) and Natural Science Foundation of Chongqing (CSTB2022NSCQ-MSX0101) .