MicroRNA-21-3p/Rcan1 signaling axis affects apoptosis of cardiomyocytes of sepsis rats

作者全名："Gong, Mingwei; Tao, Li; Li, Xia"

作者地址："[Gong, Mingwei; Tao, Li; Li, Xia] Chongqing Med Univ, Affiliated Hosp 3, Jeer Hosp, Dept Crit Care Med, Chongqing, Peoples R China; [Li, Xia] Chongqing Med Univ, Affiliated Hosp 3, Jeer Hosp, Dept Crit Care Med, 1 Shuanghu Branch Rd, Chongqing 401120, Peoples R China"

通信作者："Li, X (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Jeer Hosp, Dept Crit Care Med, 1 Shuanghu Branch Rd, Chongqing 401120, Peoples R China."

来源：GENERAL PHYSIOLOGY AND BIOPHYSICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001022183200001

JCR分区：Q4

影响因子：1.3

年份：2023

卷号：42

期号：3

开始页：217

结束页：227

文献类型：Article

关键词：Sepsis; microRNA-21-3p; Regulator of calcineurin 1; Cardiomyocytes; Apoptosis

摘要："Accumulating evidence has reported the role of microRNA-21-3p (miR-21-3p) in sepsis, and our objective was to discuss the effect of the miR-21-3p/regulator of calcineurin 1 (Rcan1) axis on cardiomyocyte apoptosis of septic rats. miR-21-3p and Rcan1 expression in myocardial tissues of lipopolysaccharide (LPS)-treated rats and LPS-treated H9c2 cardiomyocytes was determined. The influences of downregulating miR-21-3p or upregulating Rcan1 in cardiomyocyte apoptosis of LPS-treated rats and LPS-treated H9c2 cardiomyocytes were then evaluated. The target relation between Rcan1 and miR-21-3p was verified. It was observed that miR-21-3p was elevated and Rcan1 was reduced in LPS-treated rats and LPS-treated H9c2 cardiomyocytes. Downregulating miR-21-3p or upregulating Rcan1 could suppress cardiomyocyte apoptosis of LPS-treated rats and LPS-treated H9c2 cardiomyocytes. Based on the dual-luciferase activity assay, miR-21-3p was directly targeted Rcan1 in H9c2 cardiomyocytes. In the rescue experiment, the LPS+miR-21-3p inhibitor+si-Rcan1 group enhanced the apoptosis of H9c2 cardiomyocytes in comparison to the LPS+miR-21-3p inhibitor+si-NC group. Together, our findings identify that the miR-21-3p/Rcan1 axis may affect apoptosis of cardiomyocytes in sepsis, which provides a new idea for understanding the potential mechanism of sepsis."

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