Inhibition of glutamatergic trigeminal nucleus caudalis- vestibular nucleus projection neurons attenuates vestibular dysfunction in the chronic-NTG model of migraine

作者全名："Zhang, Yun; Zhang, Yixin; Wang, Yanyun; Zhang, Xiaoyan; Qin, Guangcheng; Zhang, Dunke; Chen, Lixue; Zhou, Jiying"

作者地址："[Zhang, Yun; Zhang, Yixin; Wang, Yanyun; Zhang, Xiaoyan; Zhou, Jiying] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, 1st You Yi Rd,Yu Zhong Dist, Chongqing 400016, Peoples R China; [Qin, Guangcheng; Zhang, Dunke; Chen, Lixue] Chongqing Med Univ, Lab Res Ctr, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Zhang, YX (通讯作者)，Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, 1st You Yi Rd,Yu Zhong Dist, Chongqing 400016, Peoples R China."

来源：JOURNAL OF HEADACHE AND PAIN

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001022368600001

JCR分区：Q1

影响因子：7.3

年份：2023

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Migraine; Vestibular dysfunction; Glutamate; CGRP; Neural circuit

摘要："Background Prior clinical studies suggest a shared mechanism between vestibular symptoms and migraine headache. However, the specific neuroanatomical substrate connecting vestibular symptoms with migraine remains to be largely unknown. Thus, the aim of this study was to further investigate the mechanisms that whether and how trigeminovestibular neurons produce effects on neuronal activation in vestibular nucleus (VN). Methods A chronic-NTG rat model was established by recurrent intermittent administration of nitroglycerin (NTG). Pain- and vestibular-related behaviors were assessed. To selectively inhibit the glutamatergic neurons and trigeminal nucleus caudalis (TNC) to VN projection neurons, the AAVs encoding engineered Gi-coupled hM4D receptor were administered in the TNC or VN area. Results We identify a glutamatergic projection from TNC to VN that mediates vestibular dysfunction in a chronic-NTG rat model. Inhibition of the Glutamate(TNC) neurons alleviates vestibular dysfunction in the chronic-NTG rat. Calcitonin gene-related peptide (CGRP)-expressing neurons in the VN received glutamatergic projections from TNC neurons. Silencing the glutamatergic TNC-VN projection neurons attenuates vestibular dysfunction in the chronic-NTG rat. Conclusions Together, we reveal a modulatory role of glutamatergic TNC-VN projection neurons in vestibular dysfunction of migraine."

基金机构：National Natural Science Foundation of China [82101296]; Cultivation Fund of The First Affiliated Hospital of Chongqing Medical University [201-0203-XKTS168]

基金资助正文：This study was supported by the National Natural Science Foundation of China (No:82101296 to Yixin Zhang) and the Cultivation Fund of The First Affiliated Hospital of Chongqing Medical University (No: 2-01-02-03-XKTS168 to Yixin Zhang)