Growth hormone reduces aneuploidy and improves oocytes quality by JAK2-MAPK3/1 pathway in aged mice

作者全名："Luo, Yun-Yao; Zeng, Xi; Zhu, Ling; Li, Chong; Xie, Juan; Dong, Qiang; Sun, Qing-Yuan; Huang, Guo-Ning; Li, Jing-Yu"

作者地址："[Luo, Yun-Yao; Zeng, Xi; Zhu, Ling; Li, Chong; Xie, Juan; Dong, Qiang; Huang, Guo-Ning; Li, Jing-Yu] Chongqing Med Univ, Women & Childrens Hosp, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing 400010, Peoples R China; [Luo, Yun-Yao; Zeng, Xi; Zhu, Ling; Li, Chong; Xie, Juan; Dong, Qiang; Huang, Guo-Ning; Li, Jing-Yu] Chongqing Clin Res Ctr Reprod Med, Chongqing Hlth Ctr Women & Children, Chongqing 400010, Peoples R China; [Sun, Qing-Yuan] Guangdong Second Prov Gen Hosp, Reprod Med Ctr, Guangzhou Key Lab Metab Dis & Reprod Hlth, Guangzhou 510310, Peoples R China"

通信作者："Huang, GN; Li, JY (通讯作者)，Chongqing Med Univ, Women & Childrens Hosp, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing 400010, Peoples R China.; Huang, GN; Li, JY (通讯作者)，Chongqing Clin Res Ctr Reprod Med, Chongqing Hlth Ctr Women & Children, Chongqing 400010, Peoples R China.; Sun, QY (通讯作者)，Guangdong Second Prov Gen Hosp, Reprod Med Ctr, Guangzhou Key Lab Metab Dis & Reprod Hlth, Guangzhou 510310, Peoples R China."

来源：JOURNAL OF TRANSLATIONAL MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001022442800001

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Aged oocytes; Growth hormone; Aneuploidy; Oocyte quality; MAPK3/1; JAK2

摘要："Background The global delay in women's reproductive age has raised concerns about age-related infertility. The decline in oocyte quality is a limiting factor of female fertility, yet there are currently no strategies to preserve oocyte quality in aged women. Here, we investigated the effects of growth hormone (GH) supplementation on aneuploidy of aged oocytes. Methods For the in vivo experiments, the aged mice (8-month-old) were intraperitoneally injected with GH daily for 8 weeks. For the in vitro experiments, germinal vesicle oocytes from aged mice were treated with GH during oocyte maturation. The impacts of GH on ovarian reserve before superovulation was evaluated. Oocytes were retrieved to assess oocyte quality, aneuploidy and developmental potential characteristics. Quantitative proteomics analysis was applied to investigate the potential targets of GH in aged oocytes. Results In this study, we demonstrated that GH supplementation in vivo not only alleviated the decline in oocyte number caused by aging, but also improved the quality and developmental potential of aged oocytes. Strikingly, we discovered that GH supplementation reduced aneuploidy in aged oocytes. Mechanically, in addition to improving mitochondrial function, our proteomic analysis indicated that the MAPK3/1 pathway may be involved in the reduction in aneuploidy of aged oocytes, as confirmed both in vivo and in vitro. In addition, JAK2 may also act as a mediator in how GH regulates MAPK3/1. Conclusions In conclusion, our research reveals that GH supplementation protects oocytes against aging-related aneuploidy and enhances the quality of aged oocytes, which has clinical significance for aged women undergoing assisted reproduction technology."

基金机构：National Nature Science Foundation of China [82201724]; Nature Science Foundation of Chongqing [CSTB2022NSCQ-MSX0838]; Science and Technology Project of Yuzhong District of Chongqing [20210180]

基金资助正文："AcknowledgementsThis work was supported by the National Nature Science Foundation of China (No.82201724), Nature Science Foundation of Chongqing (No. CSTB2022NSCQ-MSX0838), and the Science and Technology Project of Yuzhong District of Chongqing (No. 20210180)."