Imbalance of Vaginal Microbiota and Immunity: Two Main Accomplices of Cervical Cancer in Chinese Women

作者全名："Li, Xiaoge; Wu, Jin; Wu, Yutong; Duan, Zhaoning; Luo, Ming; Li, Ling; Li, Sijing; Jia, Ying"

作者地址："[Li, Xiaoge; Wu, Jin; Wu, Yutong; Duan, Zhaoning; Luo, Ming; Li, Ling; Li, Sijing; Jia, Ying] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Jia, Y (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF WOMENS HEALTH

ESI学科分类：　

WOS号：WOS:001022966400001

JCR分区：Q2

影响因子：2.5

年份：2023

卷号：15

期号：　

开始页：987

结束页：1002

文献类型：Article

关键词：vaginal microbiota; immune factors; cervical cancer; 16S rDNA sequencing; protein chip

摘要："Objective: To explore the correlation of female vaginal microbiota and immune factors with cervical cancer.Methods: The distribution pattern difference of vaginal microbiota of four groups of women (cervical cancer, HPV-positive CIN, HPV-positive non-CIN, and HPV-negative groups) were compared by microbial 16S rDNA sequencing. The protein chip was used to detect the composition and changes of the immune factors in the four groups.Results: Alpha diversity analysis demonstrated that the diversity of the vaginal microbiota was increased as the disease develops. Among those bacteria abundant in the vaginal microbiota, Lactobacillus, Prevotella, and Gardnerella dominate at the genus level of vaginal flora. Compared with the HPV-negative group, the differentially dominant bacteria, such as Prevotella, Ralstonia, Gardnerella and Sneathia, are enriched in the cervical cancer group. Likewise, Gardnerella, Prevotella, and Sneathia are more in the HPV-positive CIN group, while Gardnerella and Prevotella in the HPV-positive non-CIN group, respectively. In contrast, Lactobacillus and Atopobium are dominant in the HPV-negative group (LDA>4log10). The concentration of inflammatory immune factors IP-10 and VEGF-A were increased in the cervical cancer group (P < 0.05), compared with other groups.Conclusion: The occurrence of cervical cancer is related to an increase of vaginal microbiota diversity and up-regulation of inflammatory immune factor proteins. The abundance of Lactobacillus was decreased while the one of Prevotella and Gardnerella were increased in the cervical cancer group, compared with other three groups. Moreover, the IP-10 and VEGF-A were also increased in the cervical cancer group. Thus, evaluation of changes in the vaginal microbiota and these two immune factor levels might be a potential non-invasive and simple method to predict cervical cancer. Furthermore, it is significant to adjust and restore the balance of vaginal microbiota and maintain normal immune function in preventing and treating cervical cancer."

基金机构：Chongqing Medical Scientific Research Project (Joint project of Chongqing Health Commission and Science and Technology Bureau) [2021MSXM332]; Municipal Graduate Student Research Innovation Project of Chongqing [CYS21219]

基金资助正文：The study was sponsored by the Chongqing Medical Scientific Research Project (Joint project of Chongqing Health Commission and Science and Technology Bureau) (2021MSXM332) and the Municipal Graduate Student Research Innovation Project of Chongqing (CYS21219) .