Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence

作者全名："Deng, Dongmei; Xie, Juan; Tian, Yin; Zhu, Ling; Liu, Xuemei; Liu, Junxia; Huang, Guoning; Li, Jingyu"

作者地址："[Deng, Dongmei; Xie, Juan; Tian, Yin; Zhu, Ling; Liu, Xuemei; Liu, Junxia; Huang, Guoning; Li, Jingyu] Chongqing Hlth Ctr Women & Children, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing, Peoples R China; [Deng, Dongmei; Xie, Juan; Tian, Yin; Zhu, Ling; Liu, Xuemei; Liu, Junxia; Huang, Guoning; Li, Jingyu] Chongqing Med Univ, Chongqing Clin Res Ctr Reprod Med, Ctr Reprod Med, Women & Childrens Hosp, Chongqing, Peoples R China"

通信作者："Huang, GN; Li, JY (通讯作者)，Chongqing Hlth Ctr Women & Children, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing, Peoples R China.; Huang, GN; Li, JY (通讯作者)，Chongqing Med Univ, Chongqing Clin Res Ctr Reprod Med, Ctr Reprod Med, Women & Childrens Hosp, Chongqing, Peoples R China."

来源：FRONTIERS IN ENDOCRINOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001023996900001

JCR分区：Q2

影响因子：3.9

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：germinal vesicle oocyte; oocyte vitrification; fertility preservation; In vitro maturation; developmental competence

摘要："IntroductionAcquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation, particularly for cancer patients necessitating immediate treatment. Two FP approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage (GV-VI) or post-IVM at the metaphase II (MII) stage (MII-VI). The optimal method remains to be determined. MethodsIn this study, mouse oocytes were collected without hormonal stimulation and vitrified either at the GV stage or the MII stage following IVM; non-vitrified in vitro matured MII oocytes served as the control (CON). The oocyte quality and developmental competence were assessed to obtain a better method for immediate FP. ResultsNo significant differences in IVM and survival rates were observed among the three groups. Nevertheless, GV-VI oocytes exhibited inferior quality, including abnormal spindle arrangement, mitochondrial dysfunction, and early apoptosis, compared to MII-VI and CON oocytes. Oocyte vitrification at the GV stage impacted maternal mRNA degradation during IVM. In addition, the GV-VI group demonstrated significantly lower embryonic developmental competence relative to the MII-VI group. RNA sequencing of 2-cell stage embryos revealed abnormal minor zygotic genome activation in the GV-VI group. ConclusionVitrification at the GV stage compromised oocyte quality and reduced developmental competence. Consequently, compared to the GV stage, oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP."

基金机构：Chongqing Science and Technology Commission [CSTB2022NSCQ-MSX0255]; Hospital Research Project of the Women and Children's Hospital of Chongqing Medical University [2021YJQN01]; Science - Health Joint Medical Scientific Research Project of Chongqing [2021MSXM108]

基金资助正文："Funding This study was supported by the Chongqing Science and Technology Commission (CSTB2022NSCQ-MSX0255), Hospital Research Project of the Women and Children's Hospital of Chongqing Medical University (2021YJQN01), and Science - Health Joint Medical Scientific Research Project of Chongqing (2021MSXM108)."