Near-infrared fluorophore IR-61 improves the quality of oocytes in aged mice via mitochondrial protection

作者全名："Qu, Jiadan; Qin, Lifeng; Guo, Jing; Zhu, Ling; Luo, Yunyao; Li, Chong; Xie, Juan; Wang, Jiaqiang; Shi, Chunmeng; Huang, Guoning; Li, Jingyu"

作者地址："[Qu, Jiadan; Zhu, Ling; Luo, Yunyao; Li, Chong; Xie, Juan; Huang, Guoning; Li, Jingyu] Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing, Peoples R China; [Qu, Jiadan; Zhu, Ling; Luo, Yunyao; Li, Chong; Xie, Juan; Huang, Guoning; Li, Jingyu] Chongqing Hlth Ctr Women & Children, Chongqing Clin Res Ctr Reprod Med, Chongqing, Peoples R China; [Qin, Lifeng; Wang, Jiaqiang] Northeast Agr Univ, Coll Life Sci, Harbin, Peoples R China; [Guo, Jing] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun, Peoples R China; [Shi, Chunmeng] Third Mil Med Univ, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China"

通信作者："Huang, GN; Li, JY (通讯作者)，Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing, Peoples R China.; Shi, CM (通讯作者)，Third Mil Med Univ, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China."

来源：BIOMEDICINE & PHARMACOTHERAPY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001025624600001

JCR分区：Q1

影响因子：6.9

年份：2023

卷号：162

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Maternal aging; Oocyte quality; IR-61; Mitochondrial function; Mice

摘要："Maternal aging is associated with a decline in oocyte quality, which leads to the decreased fertility. Therefore, developing approaches to reduce aging-induced deterioration of oocyte quality in older women is important. Near-infrared cell protector-61 (IR-61), a novel heptamethine cyanine dye, has the potential for antioxidant effects. In this study, we found that IR-61 can accumulate in the ovaries and improved ovarian function of naturally aged mice; it also increased the oocyte maturation rate and quality by maintaining the integrity of the spindle/chromosomal structure and reducing the aneuploidy rate. In addition, the embryonic developmental competence of aged oocytes was improved. Finally, RNA-sequencing analysis indicated that IR-61 might perform the beneficial effects on aged oocytes by regulating mitochondrial function, this was confirmed by immunofluorescence analysis of mitochondrial distribution and reactive oxygen species. Taken together, our findings demonstrate that IR-61 supplementation in vivo can increase oocyte quality and protect oocytes from aginginduced mitochondrial dysfunction, and thus could improve the fertility of older women and efficiency of assisted reproductive technology."

基金机构："Natural Science Foundation of Chongqing Province [CSTB2022NSCQ-MSX0961]; National Natural Science Foundation of China [82101643, 82030056]; Chongqing Health Center for Women and Children [2020YJMS03]"

基金资助正文："We thank the research staff for their contributions to this study. This study was supported by the Natural Science Foundation of Chongqing Province (NO. CSTB2022NSCQ-MSX0961), National Natural Science Foundation of China (NO. 82101643 and NO. 82030056) and special & nbsp;research project of the Chongqing Health Center for Women and Children (NO. 2020YJMS03)."