Casein Reactivates Dopaminergic Nerve Injury and Intestinal Inflammation with Disturbing Intestinal Microflora and Fecal Metabolites in a Convalescent Parkinson's Disease Mouse Model

作者全名："Pu, Zhengjia; Liu, Shuya; Guo, Zeming; Zhang, Xuemei; Yan, Jie; Tang, Yong; Xiao, Hong; Gao, Jieying; Li, Yingli; Bai, Qunhua"

作者地址："[Pu, Zhengjia; Guo, Zeming; Zhang, Xuemei; Yan, Jie; Xiao, Hong; Gao, Jieying; Li, Yingli; Bai, Qunhua] Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Chongqing 400016, Peoples R China; [Liu, Shuya] Chongqing Inst TB Prevent & Treatment, Chongqing 400050, Peoples R China; [Tang, Yong] Chongqing Orthoped Hosp Tradit Chinese Med, Chongqing 400012, Peoples R China"

通信作者："Bai, QH (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Chongqing 400016, Peoples R China."

来源：NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001027011300001

JCR分区：Q2

影响因子：2.9

年份：2023

卷号：524

期号：　

开始页：120

结束页：136

文献类型：Article

关键词：Parkinson's disease; casein; convalescent PD mice; MPTP; gut microbiota; fecal metabolite

摘要："disease (PD) is the fastest-growing neurodegenerative disease, with pathogenic causes elusive and short of effective treatment options. Investigations have found that dairy products positively correlate with the onset of PD, but the mechanisms remain unexplored. As casein is an antigenic component in dairy prod-ucts, this study assessed if casein could exacerbate PD-related symptoms by stimulating intestinal inflammation and unbalanced intestinal flora and be a risk factor for PD. Using a convalescent PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), the results showed casein reduced motor coordination, caused gastrointestinal dysfunction, reduced dopamine content, and induced intestinal inflammation. Meanwhile, casein disturbed gut microbiota homeostasis by increasing the Firmicutes/Bacteroidetes ratio, decreasing a-diversity, and caused abnormal alterations in fecal metabolites. However, these adverse effects of casein attenu-ated much when it had hydrolyzed by acid or when antibiotics inhibited the intestinal microbiota of the mice. Therefore, our results suggested that casein could reactivate dopaminergic nerve injury and intestinal inflamma-tion and exacerbate intestinal flora disorder and its metabolites in convalescent PD mice. These damaging effects might be related to disordered protein digestion and gut microbiota in these mice. These findings will provide new insights into the impact of milk/dairy products on PD progression and supply information on dietary options for PD patients.& COPY; 2023 IBRO. Published by Elsevier Ltd. All rights reserved."

基金机构：Chongqing Medical University Student Scientific Research and Innovation Experiment Project [SRIEP202108]; Public Health Elite Project of Chongqing Medical University [GWJY2021005]

基金资助正文："We gratefully acknowledge the technical support for 16S ribosomal RNA gene sequencing and metabolite identification of Shanghai Applied Protein Technology Co., Ltd. This study was supported by the Chongqing Medical University Student Scientific Research and Innovation Experiment Project (SRIEP202108) and the Public Health Elite Project of Chongqing Medical University (GWJY2021005) ."