Universal probe-based SNP genotyping with visual readout: a robust and versatile method

作者全名："Wang, Zhongzhong; Zhang, Zhang; Luo, Wang; Wang, Luojia; Han, Xiaole; Zhao, Rong; Liu, Xin; Zhang, Jianhong; Yu, Wen; Li, Junjie; Yang, Yujun; Zuo, Chen; Xie, Guoming"

作者地址："[Wang, Zhongzhong; Zhang, Zhang; Luo, Wang; Wang, Luojia; Han, Xiaole; Zhao, Rong; Liu, Xin; Li, Junjie; Yang, Yujun; Zuo, Chen; Xie, Guoming] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid,Chinese Minist Educ, Chongqing 400016, Peoples R China; [Wang, Zhongzhong; Zhang, Zhang; Luo, Wang; Wang, Luojia; Han, Xiaole; Zhao, Rong; Liu, Xin; Li, Junjie; Yang, Yujun; Zuo, Chen; Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing 400016, Peoples R China; [Zhang, Jianhong] Chongqing Med Univ, Affiliated Hosp 1, Clin Labs, Chongqing 400016, Peoples R China; [Yu, Wen] Chongqing Univ, Canc Hosp, Chongqing 400030, Peoples R China; [Yu, Wen] Chongqing Canc Inst, Chongqing 400030, Peoples R China"

通信作者："Yang, YJ; Zuo, C; Xie, GM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid,Chinese Minist Educ, Chongqing 400016, Peoples R China.; Yang, YJ; Zuo, C; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing 400016, Peoples R China."

来源：NANOSCALE

ESI学科分类：PHYSICS

WOS号：WOS:001028683900001

JCR分区：Q1

影响因子：5.8

年份：2023

卷号：15

期号：30

开始页：12660

结束页：12669

文献类型：Article

关键词：　

摘要："Detection of single nucleotide polymorphisms (SNPs) is critical for personalized clinical diagnosis, treatment, and medication. Current clinical detection methods suffer from primer dimerization and require the redesigning of reaction systems for different targets, resulting in a time-consuming and laborious process. Here, we present a robust and versatile method for SNP typing by using tailed primers and universal small molecule probes in combination with a visualized lateral flow assay (LFA). This approach enables not only rapid typing of different targets, but also eliminates the interference of primer dimers and enhances the accuracy and reliability of the results. Our proposed universal assay has been successfully applied to the typing of four SNP loci of clinical samples to verify the accuracy and universality, and the results are consistent with those obtained by Sanger sequencing. Therefore, our study establishes a new universal ""typing formula"" using nucleic acid tags and small molecule probes that provides a powerful genotyping platform for genetic analysis and molecular diagnostics."

基金机构："National Key Ramp;D Program of China [2022YFC2603800]; National Natural Science Foundation of China [82002256]; China Postdoctoral Science Foundation [2021TQ0395]; Natural Science Foundation of Chongqing, China [cstc2021jcyj-msxmX0326]"

基金资助正文："This research work was financially supported by the National Key R&D Program of China (2022YFC2603800), the National Natural Science Foundation of China (82172369), the National Natural Science Foundation of China (82002256), the China Postdoctoral Science Foundation (2021TQ0395) and the Natural Science Foundation of Chongqing, China (cstc2021jcyj-msxmX0326)."