Dynamic effects of miR-20a-5p on hippocampal ripple energy after status epilepticus in rats

作者全名："Zhang, Xinyu; Yi, Yanjun; Cheng, Li; Chen, Hengsheng; Hu, Yue"

作者地址："[Zhang, Xinyu; Yi, Yanjun; Cheng, Li; Chen, Hengsheng; Hu, Yue] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Neurol,Chongqing Key Lab Pediat,Childrens Hos, Minist Educ,Key Lab Child Dev & Disorders, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China; [Zhang, Xinyu; Yi, Yanjun; Cheng, Li; Chen, Hengsheng] Chongqing Med Univ, Pediat Res Inst, Childrens Hosp, Chongqing 400014, Peoples R China"

通信作者："Hu, Y (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Neurol,Chongqing Key Lab Pediat,Childrens Hos, Minist Educ,Key Lab Child Dev & Disorders, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China."

来源：EXPERIMENTAL BRAIN RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001031330800001

JCR分区：Q4

影响因子：1.7

年份：2023

卷号：241

期号：8

开始页：2097

结束页：2106

文献类型：Article

关键词：miR-20a-5p; Valproate sodium; Status epilepticus; Ripple oscillation

摘要："To determine the dynamic effects of miR-20a-5p on hippocampal ripple energy in rats after status epilepticus (SE). A lithium pilocarpine (LiCl-PILO)-induced rat model of status epilepticus (SE) was established, and the rats were divided into the normal control (Control, CTL), epileptic control (PILO), valproic acid (VPA + PILO), miR-20a-5p overexpression lentivirus vector (miR + PILO), sponges blocking lentivirus vector (Sponges + PILO), and scramble sequence negative control (Scramble + PILO) groups (n = 6). Electroencephalograms (EEGs) were used to analyze changes in hippocampal ripple energy before and after SE. Quantitative polymerase chain reaction (q-PCR) analysis showed that miR-20a-5p levels gradually increased after miR-20a-5p overexpression lentivirus vector injection into the lateral ventricle, and the miR-20a-5p levels were significantly higher than that in CTL group on days 7 and 36 (P < 0.001). The miR-20a-5p levels decreased significantly on days 7 and 36 after blocking by sponges lentivirus vector injected into the lateral ventricle (P < 0.001). After injection of PILO, the average ripple energy expression in each group gradually increased, and reached the peak before chloral hydrate injection (compared with 1 day before SE, P < 0.05). The ripple energy in the VPA + PILO and Sponges + PILO groups was significantly lower than that in the PILO group at 60 min and 70 min after PILO injection and before chloral hydrate injection (P < 0.05), and maintained lower until 2 h after chloral hydrate injection in VPA + PILO (P < 0.05). Compared with the VPA + PILO group, the mean ripple energy of the Sponges + PILO group had no difference at all time points (P & GE; 0.05). After SE, ripple distribution of space and energy is closely related to the occurrence of epilepsy. Inhibition of miR20a-5p expression can downregulate ripple oscillation energy during seizure."

基金机构："National Natural Science Foundation of China [81971215, 81301124]; Chinese Anti-Epilepsy Association Epilepsy Research Foundation-UCB Foundation [2019036]"

基金资助正文："This work was supported by National Natural Science Foundation of China (81971215, 81301124). Chinese Anti-Epilepsy Association Epilepsy Research Foundation-UCB Foundation (2019036)."