Metabolomic profiling identifies hair as a robust biological sample for identifying women with cervical cancer

作者全名："Ran, Rui; Zhong, Xiaocui; Yang, Yang; Tang, Xianglan; Shi, Manlin; Jiang, Xingwei; Lin, Anping; Gan, Xiaoling; Yu, Tinghe; Hu, Lina; Dong, Xiaojing; Han, Ting-Li"

作者地址："[Ran, Rui; Zhong, Xiaocui; Tang, Xianglan; Shi, Manlin; Jiang, Xingwei; Lin, Anping; Gan, Xiaoling; Yu, Tinghe; Hu, Lina; Dong, Xiaojing; Han, Ting-Li] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 2, Chongqing 400016, Peoples R China; [Yang, Yang] Chongqing Med Univ, Dept Obstet, Affiliated Hosp 1, Chongqing 400716, Peoples R China; [Han, Ting-Li] Univ Auckland, Liggins Inst, Auckland 1023, New Zealand"

通信作者："Han, TL (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 2, Chongqing 400016, Peoples R China.; Han, TL (通讯作者)，Univ Auckland, Liggins Inst, Auckland 1023, New Zealand."

来源：MEDICAL ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001035292000001

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：40

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：Cervical cancer; Hair specimen; Metabolomics; Biomarker discovery; Correlation analysis

摘要："Metabolomics serves as a useful tool for identifying biomarkers of disease and uncovering pathogenic mechanisms. However, most metabolomic studies use biological fluids such as blood and urine as biospecimens, which could be dramatically influenced by daily activities and dietary variation, resulting in measurement fluctuations. In contrast, hair may serve as a robust source of stable longitudinal metabolite information. Here, we conducted a pilot study to investigate the possibility of using hair as a biospecimen for the metabolomic analysis of cervical cancer. Hair, plasma, urine, and cervical tissue samples from cervical cancer and benign tumor patients were collected. Biospecimens were then tested using a gas chromatography-mass spectrometry-based metabolomic platform. The expressions of enzymatic genes related to metabolic changes were validated using qPCR. Statistical analyses were calculated via the R-console platform. Metabolite profiles in both hair and cervical tissue samples were significantly different between cancer and control groups, while no difference was observed in plasma and urine samples. Further analysis showed that most of the altered metabolites in hair were upregulated, and they had a negative correlation with those in the cervical tissue. Eight common metabolites showed an area under the Receiver Operating Characteristic curve greater than 0.95. These metabolites primarily participated in amino acid metabolism, cofactor synthesis, ferroptosis, and glycolysis. The gene expressions (IDH1, OGDH, GLUD1, ENO1, GSS, and GPX4) associated with the shortlisted metabolic pathways were also upregulated. Our study is the first to reveal metabolomic changes of hair in cervical cancer patients and demonstrates the potential for the hair metabolome to be used for biomarker identification in cervical cancer."

基金机构："National Natural Science Foundation of China [81871185, 81961128004]; Natural Science Foundation of Chongqing [CSTB2022NSCQ-MSX0754]; Kuanren Talents Programs of the Second Affiliated Hospital of Chongqing Medical University"

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grant Nos. 81871185, 81961128004), Natural Science Foundation of Chongqing (Grant Nos. CSTB2022NSCQ-MSX0754), Kuanren Talents Programs of the Second Affiliated Hospital of Chongqing Medical University."