Genomic features and virulence characteristics of a rare Burkholderia thailandensis strain causing human infection

作者全名："Li, Jin; Zhong, Qiu; Li, Jian; Chong, Hui-Min; Wang, Li-Xin; Xing, Yun; Lu, Wei-Ping"

作者地址："[Li, Jin; Zhong, Qiu; Li, Jian; Chong, Hui-Min; Wang, Li-Xin; Xing, Yun; Lu, Wei-Ping] Army Med Univ, Mil Med Univ 3, Daping Hosp, Dept Lab Med, Chongqing 400042, Peoples R China; [Li, Jin] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Lu, WP (通讯作者)，Army Med Univ, Mil Med Univ 3, Daping Hosp, Dept Lab Med, Chongqing 400042, Peoples R China."

来源：JOURNAL OF MEDICAL MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001039583700002

JCR分区：Q3

影响因子：2.4

年份：2023

卷号：72

期号：5

开始页：　

结束页：　

文献类型：Article

关键词：B; thailandensisBPM; whole-genome sequencing; comparative genomics analysis; genomic features; virulence characteristics

摘要："Introduction. Burkholderia thailandensis is a clinically rare opportunistic pathogen in the genus Burkholderia, and the genomic features and virulence characteristics of B. thailandensis strains that cause human infection remain unclear.Gap Statement. B. thailandensis strains with different virulence induce different host innate immune responses in vitro.Aim. This work aimed to understand the sequence diversity, phylogenetic relationship, and virulence of B. thailandensis BPM causing human infection.Methodology. The comparative molecular and genomic analyses, and mouse infection studies were applied to analyse the virulence and genomic features of B. thailandensis BPM originating from China. Results. The whole genome sequence analysis showed that the genomes of BPM and other avirulent B. thailandensis strains were broadly similar, comprising two highly syntenic chromosomes with comparable numbers of coding regions (CDs), protein family distributions, and horizontally acquired genomic islands. By examining species-specific genomic regions, we obtained molecular explanations for previously known differences in virulence and discovered the potential specific virulence-associated genes of BPM, which likely work together to confer the virulence of BPM. Significantly reduced LD50 and survival rates during mouse infection experiments were found in BPM compared to the avirulent B. thailandensis E264 (BtE264).Conclusion. Taken together, the results of this study provide basic information on the genomic features and virulence charac-teristics of the virulent B. thailandensis strain BPM, which is helpful for understanding its evolution as it relates to pathogenesis and environmental adaptability."

基金机构：National Natural Science Foundation of China [82072348]; Youth Project of the National Natural Science Foundation of China [82002113]

基金资助正文：Funding information This study was supported by the National Natural Science Foundation of China (82072348) and the Youth Project of the National Natural Science Foundation of China (82002113) .