Quercetin promotes autophagy to alleviate cigarette smoke-related periodontitis

作者全名："Yu, Jinrui; Jing, Zheng; Shen, Danfeng; Yang, Mingcong; Liu, Kehao; Xiang, Kai; Zhou, Chongjing; Gong, Xuerui; Deng, Yangjia; Li, Yuzhou; Yang, Sheng"

作者地址："[Yu, Jinrui; Jing, Zheng; Shen, Danfeng; Yang, Mingcong; Liu, Kehao; Xiang, Kai; Zhou, Chongjing; Gong, Xuerui; Deng, Yangjia; Li, Yuzhou; Yang, Sheng] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China; [Jing, Zheng; Li, Yuzhou; Yang, Sheng] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Jing, Zheng; Li, Yuzhou; Yang, Sheng] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Li, Yuzhou; Yang, Sheng] 426 Songshi North Rd, Chongqing 401147, Peoples R China"

通信作者："Li, YZ; Yang, S (通讯作者)，426 Songshi North Rd, Chongqing 401147, Peoples R China."

来源：JOURNAL OF PERIODONTAL RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001039649700001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：autophagy; cigarette smoke; oxidative stress; periodontal ligament cells; periodontitis; quercetin

摘要："Background and ObjectivesCigarette smoking has been reported as an independent risk factor for periodontitis. Tobacco toxins affect periodontal tissue not only locally but also systemically, leading to the deterioration and recurrence of periodontitis. However, the mechanism of cigarette smoke-related periodontitis (CSRP) is unclear and thus lacks targeted treatment strategies. Quercetin, a plant-derived polyphenolic flavonoid, has been reported to have therapeutic effects on periodontitis due to its documented antioxidant activity. This study aimed to evaluate the effects of quercetin on CSRP and elucidated the underlying mechanism. MethodsThe cigarette smoke-related ligature-induced periodontitis mouse model was established by intraperitoneal injection of cigarette smoke extract (CSE) and silk ligation of bilateral maxillary second molars. Quercetin was adopted by gavage as a therapeutic strategy. Micro-computed tomography was used to evaluate the alveolar bone resorption. Immunohistochemistry detected the oxidative stress and autophagy markers in vivo. Cell viability was determined by Cell Counting Kit-8, and oxidative stress levels were tested by 2,7-dichlorodihydrofluorescein diacetate probe and lipid peroxidation malondialdehyde assay kit. Alkaline phosphatase and alizarin red staining were used to determine osteogenic differentiation. Network pharmacology analysis, molecular docking, and western blot were utilized to elucidate the underlying molecular mechanism. ResultsAlveolar bone resorption was exacerbated and oxidative stress products were accumulated during CSE exposure in vivo. Oxidative stress damage induced by CSE caused inhibition of osteogenic differentiation in vitro. Quercetin effectively protected the osteogenic differentiation of human periodontal ligament cells (hPDLCs) and periodontal tissue by upregulating the expression of Beclin-1 thus to promote autophagy and reduce oxidative stress damage. ConclusionOur results established a role of oxidative stress damage and autophagy dysfunction in the mechanism of CSE-induced destruction of periodontal tissue and hPDLCs, and provided a potential application value of quercetin to ameliorate CSRP."

基金机构："National Natural Science Foundation of China [82171010, 82001103, 82270962]; Natural Science Foundation of Chongqing [cstc2021jcyj-jqX0028, cstc2020jcyj-msxmX0131, CSTB2022NSCQ-MSX0925]; CQMU Program for Youth Innovation in Future Medicine [W0079]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN202200434]"

基金资助正文："ACKNOWLEDGEMENTS This work was supported by the National Natural Science Foundation of China (Grant No. 82171010, 82001103, 82270962), the Natural Science Foundation of Chongqing (Grant No. cstc2021jcyj-jqX0028, cstc2020jcyj-msxmX0131, CSTB2022NSCQ-MSX0925), the CQMU Program for Youth Innovation in Future Medicine (No. W0079), and the Scientific and Technological Research Program of Chongqing Municipal Education Commission (Grant No. KJQN202200434)."