Long non-coding RNA AC245100.4 activates the PI3K/AKT pathway to promote PCa cell proliferation by elevating PAR2

作者全名："Zhang, Ke; Liu, Chi; Hu, Changbin; Lin, Ping; Qi, Qi; Jia, Huizhen; Tang, Jiebing; Yu, Xiaoguang"

作者地址："[Tang, Jiebing] Harbin Med Univ, Dept Gastrointestinal Med Oncol, Canc Hosp, Harbin, Heilongjiang, Peoples R China; [Zhang, Ke; Liu, Chi; Hu, Changbin; Lin, Ping; Qi, Qi; Jia, Huizhen; Tang, Jiebing; Yu, Xiaoguang] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin 150086, Heilongjiang, Peoples R China; [Hu, Changbin] Chongqing Med Univ, Univ Town Hosp, Dept Rehabil, Chongqing 40016, Peoples R China; [Tang, Jiebing] Harbin Med Univ, Dept Gastrointestinal Med Oncol, Canc Hosp, Harbin 150086, Heilongjiang, Peoples R China"

通信作者："Tang, JB; Yu, XG (通讯作者)，Harbin Med Univ, Dept Biochem & Mol Biol, Harbin 150086, Heilongjiang, Peoples R China.; Tang, JB (通讯作者)，Harbin Med Univ, Dept Gastrointestinal Med Oncol, Canc Hosp, Harbin 150086, Heilongjiang, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001039850200001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：6

开始页：　

结束页：　

文献类型：Article

关键词：Prostate cancer; LncRNA AC245100; 4; Proliferation; PAR2; PI3K; AKT pathway

摘要："Background: Prostate cancer (PCa) is among the most generally diagnosed cancers in males. A long non-coding RNA (lncRNA) called AC245100.4 has been discovered and linked to PCa carcino-genesis. However, its specific and potential mechanism is uncertain in PCa. In this research, we investigated the role of AC245100.4 in cell proliferation and the underlying mechanism in PCa cells.Methods: qRT-PCR assays were utilized to detect AC245100.4 expression and confirm its down-stream target. The pathways related to AC245100.4 were identified by RAP-MS. PCa cell pro-liferation was experimented by Cell Counting Kit-8 and Colony formation assays. Western blot was performed to detect PAR2, AKT, p-AKT, Cyclin D1 and PCNA expression.Results: AC245100.4/PAR2 overexpression promotes PCa cell proliferation and the opposite re-sults are obtained after AC245100.4/PAR2 knockdown. Mechanistically, we found that PAR2 is confirmed as the AC245100.4 downstream target and AC245100.4 promotes PCa cell prolifera-tion by regulating PAR2. AC245100.4 promotes PCa cell proliferation via PI3K/AKT pathway. Rescue assays validated that PAR2 knockdown reversed the impact of AC245100.4 over -expression on increasing p-AKT protein levels. Conclusion: This research revealed that AC245100.4 enhances cell proliferation in PCa cells through modulating the PAR2/PI3K/AKT axis, which may offer novel tumor markers and po-tential therapeutic targets for PCa."

基金机构：Haiyan Research Fund of Harbin Medical University Cancer Hospital [JJMS2021-13]

基金资助正文：Funding This study was supported by Haiyan Research Fund of Harbin Medical University Cancer Hospital (No. JJMS2021-13) .