Dual-functional borosilicate glass (BSG) delivery implant for osteomyelitis treatment and bone regeneration

作者全名："Zhang, Hao; Li, Li; Chu, Lei; Huang, Jing; Chen, Xiaochen; Zhuo, Xianglong; Wang, Yulin; Honiball, John Robert; Wang, Bin; Chen, Wei; Lu, William W.; Wang, Deping; Li, Bing; Cui, Xu; Pan, Haobo"

作者地址："[Zhang, Hao; Wang, Bin; Lu, William W.; Cui, Xu; Pan, Haobo] Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Human Tissues & Organs Degenerat, Shenzhen 518055, Guangdong, Peoples R China; [Li, Li; Huang, Jing; Zhuo, Xianglong; Wang, Yulin; Li, Bing] Guangxi Med Univ, Affiliated Hosp 4, Liuzhou Workers Hosp, Dept Orthoped, Liuzhou 545000, Guangxi, Peoples R China; [Li, Li; Honiball, John Robert; Lu, William W.] Univ Hong Kong, Dept Orthopaed & Traumatol, Hong Kong 999077, Peoples R China; [Chu, Lei] Chongqing Med Univ, Affiliated Hosp 2, Dept Orthopaed, Chongqing 400010, Peoples R China; [Chen, Xiaochen; Wang, Deping] Tongji Univ, Sch Mat Sci, Shanghai 201804, Peoples R China; [Chen, Xiaochen; Wang, Deping] Tongji Univ, Sch Engn, Shanghai 201804, Peoples R China; [Chen, Wei] Hebei Med Univ, Hosp 3, Dept Orthopaed Surg, Shijiazhuang 050051, Peoples R China; [Chen, Wei] Hebei Med Univ, Hosp 3, NHC Key Lab Intelligent Orthopead Equipment, Shijiazhuang 050051, Peoples R China"

通信作者："Cui, X; Pan, HB (通讯作者)，Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Human Tissues & Organs Degenerat, Shenzhen 518055, Guangdong, Peoples R China.; Li, B (通讯作者)，Guangxi Med Univ, Affiliated Hosp 4, Liuzhou Workers Hosp, Dept Orthoped, Liuzhou 545000, Guangxi, Peoples R China."

来源：COMPOSITES PART B-ENGINEERING

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001041160100001

JCR分区：Q1

影响因子：12.7

年份：2023

卷号：259

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Borosilicate glass; Drug release kinetics; Signaling pathway; Osteomyelitis; Bone regeneration

摘要："Effective treatment of osteomyelitis remains to be clinical challenge due to the recurrent and persistent nature of the disease. Studies have shown that borate glasses can serve as implants for local drug delivery in treating osteomyelitis, as well as aid in regenerating bone defects in vivo. However, both the kinetics of drug release from borate glass-based implants and the underlying molecular mechanism for promoting bone regeneration remain unclear. Here, delivery implants with varying amounts of antibiotics were fabricated by encapsulating genta-micin sulfate (GS) into a chitosan glue and applying it to borosilicate glass (BSG). Upon immersion in phosphate buffered saline (PBS), delivery implants degraded and converted into hydroxyapatite (HA). All delivery implants released GS sustainably over a period of around 25 days in both PBS and K2HPO4 solutions and displayed efficacious inhibition of E. coli and S. aureus in vitro. GS release occurred through diffusion for inhibition whereby Fickian diffusion was revealed to be the predominant release kinetic. Ions (B, Ca, P and Si) released from BSG supported the proliferation, differentiation, osteogenic gene expression and protein secretion of human bone marrow mesenchymal stem cells (hBMSCs) in vitro. When implanted in a rabbit tibial osteomyelitis model, osteomyelitis in the experimental group with delivery implants was effectively cured. Meanwhile, delivery im-plants have significant osteogenesis-promoting effects after implantation time of 12-weeks in vivo. Implantation of BSG-based drug delivery implants developed in this study may provide a promising method for treating osteomyelitis as well as for facilitating efficient bone regeneration."

基金机构："National Key Ramp;D Program of China [2021YFC2400500, 2018YFC1106300]; National Natural Science Foundation of China [U22A20357, 52072398, 31870956, 81860385, 32161160327, U2001221, 52172286]; National Natural Science Foundation of China (NSFC) /Research Grants Council (RGC) [N_HKU 753/21]; Research Grants Council (RGC) /General Research Fund (GRF) [17101821]; Frontier Science Key Research Programs of CAS [QYZDB-SSW-JSC030]; Shenzhen Significant Strategy Layout Project [JCYJ20200109114620793]; Shenzhen-HongKong Joint Innovation Project [SGDX2019091716460172]; Natural Science Foundation of Guangxi Province [2022GXNSFAA035472, 2020GXNSFBA297154]; Natural Science Foundation of Chongqing [cstc2021jcyj-msxmX0134]"

基金资助正文："Acknowledgments This work was supported by the National Key R & D Program of China (Grant No. 2021YFC2400500 and 2018YFC1106300) , the National Natural Science Foundation of China (Grant No. U22A20357, 52072398, 31870956, 81860385, 32161160327, U2001221 and 52172286) , the National Natural Science Foundation of China (NSFC) /Research Grants Council (RGC) (Grant No. N_HKU 753/21) , the Research Grants Council (RGC) /General Research Fund (GRF) (Grant No. 17101821) , the Frontier Science Key Research Programs of CAS (Grant No. QYZDB-SSW-JSC030) , the Shenzhen Significant Strategy Layout Project (Grant No. JCYJ20200109114620793) , Shenzhen-HongKong Joint Innovation Project (Grant No. SGDX2019091716460172) , the Natural Science Foundation of Guangxi Province (Grant No. 2022GXNSFAA035472 and 2020GXNSFBA297154) , the Natural Science Foundation of Chongqing (Grant No. cstc2021jcyj-msxmX0134) ."