CircPGPEP1 promotes the growth and metastasis of breast cancer cells by targeting miR-30b

作者全名："Tan, Yongjia; Wang, Xiang"

作者地址："[Tan, Yongjia] Chongqing Beaucare Clin, Chongqing 400010, Peoples R China; [Tan, Yongjia] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Gen Surg, Chongqing 400038, Peoples R China; [Wang, Xiang] Chongqing Med Univ, Phys Examinat Ctr, Affiliated Hosp 3, Chongqing 401120, Peoples R China"

通信作者："Wang, X (通讯作者)，Chongqing Med Univ, Phys Examinat Ctr, Affiliated Hosp 3, Chongqing 401120, Peoples R China."

来源：MOLECULAR & CELLULAR TOXICOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001041450200002

JCR分区：Q4

影响因子：1.1

年份：2023

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期号：　

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结束页：　

文献类型：Article; Early Access

关键词：circPGPEP1; miR-30b; ITGB3; Proliferation; Invasion; Migration; Apoptosis

摘要："BackgroundCircular RNAs (circRNAs) and microRNAs (miRNAs) are closely associated with tumorigenesis. Breast cancer is a heterogeneous disease with subtypes. It is one of the leading causes of death in women, and dysregulation of various circRNAs and miRNAs are involved in the development of breast cancer. Furthermore, circPGPEP1(hsa_circ_0050102) could function as a tumor promoter. However, the potential mechanisms of circPGPEP1-modulated signaling in breast cancer are still unknown.ObjectiveThe aim of this study was to identify the detailed roles of circPGPEP1 during tumor progression. The expression profiles of circPGPEP1 and miR-30b in breast cancer tissues and cells were examined using RT-qPCR. The protein levels of ITGB3 in MCF-7 and T47D cells transfected with miR-30b mimics were determined by western blotting. Additionally, the proliferation, invasion and migration of transfected breast cancer cells were evaluated using CCK8 and Transwell assays. Moreover, the apoptotic rates of treated cancer cells were measured by flow cytometry. Luciferase reporter assay was used to explore the interaction between miR-30b and circPGPEP1/ITGB3.ResultsOur results revealed upregulation of circPGPEP1 in breast cancer tissues and cell lines, which was associated with poor prognosis in the patients. Knockdown of circPGPEP1 inhibited the proliferation, invasion and migration of cancer cells, while cell apoptosis was promoted. The expression of miR-30b was increased but the levels of ITGB3 were decreased following the knockdown of circPGPEP1. Furthermore, miR-30b was negatively correlated with circPGPEP1 and ITGB3 in breast cancer tissues. In addition, miR-30b mimics suppressed the growth of breast cancer cells, whereas cell apoptosis was enhanced. These effects were reversed by the overexpression of ITGB3. Furthermore, miR-30b mimics could abrogate the biological behavior changes caused by oe-circPGPEP1.ConclusionCircPGPEP1 could function as a tumor promoter by targeting miR-30b and ITGB3. The circPGPEP1/miR-30b/ITGB3 axis may regulate the proliferation, invasion, migration and apoptosis of breast cancer cells, which could be novel prognostic and therapeutic target."

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