An ultrasensitive solid-state ECL biosensor based on synergistic effect between Zn-NGQDs and porphyrin-based MOF as "on-off-on" platform

作者全名："Dai, Chenglin; Gan, Yongjun; Qin, Junchuan; Ma, Lianju; Liu, Qian; Huang, Liyun; Yang, Zengtao; Zang, Guangchao; Zhu, Shu"

作者地址："[Dai, Chenglin; Gan, Yongjun; Qin, Junchuan; Ma, Lianju; Liu, Qian; Huang, Liyun; Zang, Guangchao; Zhu, Shu] Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Pharm & Chem, Chongqing 401331, Peoples R China; [Dai, Chenglin; Gan, Yongjun; Qin, Junchuan; Ma, Lianju; Liu, Qian; Huang, Liyun; Zang, Guangchao; Zhu, Shu] Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Tissue & Cell Biol, Chongqing 401331, Peoples R China; [Yang, Zengtao] Chongqing Med Univ, Coll Biomed Engn, Chongqing 401331, Peoples R China"

通信作者："Zang, GC; Zhu, S (通讯作者)，Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Pharm & Chem, Chongqing 401331, Peoples R China.; Zang, GC; Zhu, S (通讯作者)，Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Tissue & Cell Biol, Chongqing 401331, Peoples R China."

来源：COLLOIDS AND SURFACES B-BIOINTERFACES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001042281100001

JCR分区：Q1

影响因子：5.4

年份：2023

卷号：226

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemiluminesence; Synergistic effect; Regulated metal-organic framework lumino phores; Porphyrin derivative; miRNA 24

摘要："To develop an ultra-sensitive solid-state electrochemiluminescence (ECL) biosensor for detection of miRNA 24, three different forms of porphyrin metal-organic framework (MOF) nanomaterials with good biocompatibility were synthesized through small molecule ligand modulation. We investigated various properties of synthesized MOFs in the presence of different small molecule ligands. The as-obtained 2D MOF nanodisk exhibited high ECL intensity and outstanding stability in the presence of a co-reactant at low concentrations. We also synthesized zinc-based quantum dots (Zn-NGQDs) with excellent photovoltaic properties by doping zinc dithiothreitol (DTT-Zn) into quantum dots. Accordingly, an enzyme-free solid-state ECL biosensor for miRNA 24 based on the ""on-off-on"" signal conversion strategy was created. Dependent on the synergy between the luminophor 2D MOF and Zn-NGQDs, the biosensor achieves a wide linear range from 1.00 x 10(-16) to 1.00 x 10(-10) mol center dot L-1 and an exceedingly low detection limit of 0.03 fM. Furthermore, the ECL biosensor exhibits outstanding selectivity, repeatability, and stability. The method has great potential for investigating sensitive detection models for various biomolecules and the design of highly efficient MOF luminescent materials."

基金机构："Scientific~and Technological Research Program of Chongqing Municipal Education Commission [KJQN202200426]; CQMU Program for Youth Innovation in Future Medicine [W0015]; Innovative Talent Promotion Program in Lab Teaching amp; Management Centre [LTMC201903, LTMC202104]; Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University [LTMCMTS202218]; Project of Tutorial System of Medical Undergraduate in Lab Teaching amp; Management Centre; [202257]; [LTMCMTS202121]"

基金资助正文："The authors gratefully acknowledge Project Supported by Scientific & nbsp;and Technological Research Program of Chongqing Municipal Education Commission (Grant No. KJQN202200426), CQMU Program for Youth Innovation in Future Medicine (W0015), Innovative Talent Promotion Program in Lab Teaching & Management Centre (LTMC201903, LTMC202104), The Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Centre (LTMCMTS202121, LTMCMTS202218) and The Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University (202257)."