"Methylated tumor suppressor gene SCARA5 inhibits the proliferation, migration and invasion of nasopharyngeal carcinoma"

作者全名："Jiang, Xianyao; Jiang, Yu; An, Deqiang; Jiang, Xiaocong; Zhou, Shitong; Liu, Yijun; Tian, Rui; Li, Zhuoqing; Zhao, Xunping; Xiang, Tingxiu; Ji, Ping; Yang, Yucheng"

作者地址："[Jiang, Xianyao; Jiang, Yu; An, Deqiang; Jiang, Xiaocong; Zhou, Shitong; Liu, Yijun; Zhao, Xunping; Yang, Yucheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Tian, Rui; Li, Zhuoqing; Xiang, Tingxiu] Chongqing Med Univ, Affiliated Hosp 1, Key Lab Mol Oncol & Epigenet, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Ji, Ping] Chongqing Med Univ, Stomatol Hosp, 7 Shangqingsi Rd, Chongqing 400015, Peoples R China; [Zhou, Shitong] Chongqing Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, 118 Xingguang Ave, Chongqing 401147, Peoples R China"

通信作者："Yang, YC (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, 1 Youyi Rd, Chongqing 400016, Peoples R China.; Ji, P (通讯作者)，Chongqing Med Univ, Stomatol Hosp, 7 Shangqingsi Rd, Chongqing 400015, Peoples R China."

来源：EPIGENOMICS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001043495500001

JCR分区：Q2

影响因子：3

年份：2023

卷号：15

期号：11

开始页：635

结束页：650

文献类型：Article

关键词：apoptosis; caspase 3; HSPA2; methylation; nasopharyngeal carcinoma; SCARA5

摘要："Background: SCARA5 may play an important role in nasopharyngeal carcinoma. Materials & methods: PCR and immunohistochemistry were used to detect the expression and promoter methylation of SCARA5. Cell proliferation assays, spheroid culture, flow cytometry analysis, Transwell assays and xenotransplantation tests were utilized to determine the functional effects of SCARA5. RNA-sequencing, western blotting, immunofluorescence and dual-luciferase reporter assays were used to assess SCARA5-mediated outcomes. Results: SCARA5 was downregulated by promoter methylation. Overexpression of SCARA5 inhibited cell migration, invasion and proliferation. SCARA5 enhanced nasopharyngeal carcinoma cell sensitivity to chemotherapy with cisplatin and 5-fluorouracil. SCARA5 drives tumor apoptosis by downregulating HSPA2. Conclusion: SCARA5 may be a useful clinical marker in nasopharyngeal carcinoma. Tweetable abstractPromoter methylation suppressed SCARA5 translation in nasopharyngeal carcinoma (NPC). SCARA5 suppressed NPC cell proliferation by promoting G0/G1 phase arrest and cell apoptosis by suppressing HSPA2 expression. Meanwhile, SCARA5 suppressed sphere-forming capacity, migration and invasion of NPC cells."

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