NCAPG stimulates lung adenocarcinoma cell stemness through aerobic glycolysis

作者全名："Zhang, Zuwang; Qi, Dongdong; Liu, Xun; Kang, Poming"

作者地址："[Zhang, Zuwang; Qi, Dongdong; Liu, Xun; Kang, Poming] Chongqing Med Univ, Univ Town Hosp, Dept Thorac Surg, Chongqing, Peoples R China; [Kang, Poming] Chongqing Med Univ, Univ Town Hosp, Dept Thorac Surg, 55 Middle Univ Rd, Chongqing 401331, Peoples R China"

通信作者："Kang, PM (通讯作者)，Chongqing Med Univ, Univ Town Hosp, Dept Thorac Surg, 55 Middle Univ Rd, Chongqing 401331, Peoples R China."

来源：CLINICAL RESPIRATORY JOURNAL

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001044194300001

JCR分区：Q3

影响因子：1.9

年份：2023

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期号：　

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结束页：　

文献类型：Article; Early Access

关键词：cell stemness; glycolysis; lung adenocarcinoma; NCAPG

摘要："BackgroundCancer stem cells are pivotal in cancer progression and therapy, including lung adenocarcinoma (LUAD). High NCAPG level is implicated in malignant tumorigenesis, but investigations on NCAPG and LUAD stem cells are warranted. Hence, projecting the impact of NCAPG on cell stemness and the targeted therapy for LUAD is of the essence. MethodsBioinformatics analyzed NCAPG expression in LUAD tissues. qRT-PCR assayed NCAPG expression in LUAD cells. CCK-8 assessed cell viability and cell sphere-forming assay measured sphere-forming ability. Western blot assessed expression of stem cell-related markers (CD133, CD44, Oct-4) and specific genes (HK2, PKM2, LDHA) related to glycolysis metabolism pathway. Cellular glycolytic capacity was assayed by glycolytic metabolites pyruvic acid, lactate, citrate, and malate assay kits, and extracellular acidification rate and oxygen consumption rate analyzers. ResultsNCAPG was upregulated in LUAD and enriched in the aerobic glycolysis pathway, and its expression was positively correlated with that of glycolytic marker genes. Cell function assays revealed that NCAPG stimulated proliferation, stemness, and glycolytic activity of LUAD cells. Rescue experiments unveiled that 2-DG (glycolysis inhibitor) was able to reverse the stimulative impact of NCAPG overexpression on proliferation, stemness, and glycolytic activity of LUAD cells. ConclusionNCAPG stimulated LUAD cell stemness through activation of glycolysis pathway. NCAPG may be possible biomarker for diagnosis and target for treatment of LUAD."

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