Study on antitumor activity of three ruthenium arene complexes in vitro

作者全名："Peng, Xiaolong; Tang, Qiang; Zhu, Huiyun; Bai, Lijuan; Zhao, Hua; Chen, Yongjie"

作者地址："[Peng, Xiaolong; Tang, Qiang; Zhu, Huiyun; Bai, Lijuan; Zhao, Hua; Chen, Yongjie] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"

通信作者："Chen, YJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China."

来源：JOURNAL OF INORGANIC BIOCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001045165500001

JCR分区：Q1

影响因子：3.8

年份：2023

卷号：247

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Binuclear ruthenium; Anticancer; Singlet oxygen; Photocleavage of DNA; Long-wave light irradiation

摘要："Three ruthenium arene complexes, namely {[(& eta;6-p-cymene)Ru(Cl)]2(dpb)}(PF6)2 (1), [(& eta;6-p-cymene)Ru(dpb)Cl] (PF6) (2) and [(& eta;6-p-cymene) Ru(dpb)py](PF6) (3) (dpb = 2,3-bis(2-pyridyl)benzo-quinoxaline, py = pyridine), were synthesized and their antitumor properties were introduced. Complexes 1-3 were characterized by 1H NMR, MS, and elemental analysis. As a binuclear ruthenium structure, the absorption of metal ligand electron transfer (MLCT) of 1 extended to 700 nm. Complex 1 was significantly hydrolyzed under dark conditions. The cytotoxicity in vitro study showed that complexes 1 and 2 are more toxic to human lung cancer cells (A549) and human cervial cancer cells (Hela) than cisplatin. Moreover, there was almost no cross-resistance between complex 1-2 and cisplatin. Under the irradiation at 478 nm, complexes 1-3 all produced singlet oxygen (1O2), and the 1O2 quantum yield of complex 1 in PBS is the highest among complexes 1-3. Complex 1 also produced 1O2 under 600 nm light irradiation. DNA gel electrophoresis showed that 1 caused the photocleavage of plasmid DNA. The hydrolysis rate of complex 1 was accelerated under light (& lambda; > 600 nm). And the phototoxicity of complex 1 to Hela cells under light (& lambda; > 600 nm) was much greater than its dark toxicity, which may be due to its generation of 1O2 and the promotion of its hydrolysis under long-wave light irradiation."

基金机构：National Natural Science Foun-dation of China [21701018]; Science and Technology Planning Project of Yuzhong District of Chongqing City [20200125]; Discipline Talent Training Program of College of Pharmacy [YXY2020SDTR01]

基金资助正文："This research was supported by the National Natural Science Foun-dation of China (21701018) , the Science and Technology Planning Project of Yuzhong District of Chongqing City (Grant No. 20200125) , and the Discipline Talent Training Program of College of Pharmacy (YXY2020SDTR01) ."