"Ferulic acid, ligustrazine, and tetrahydropalmatine display the anti-proliferative effect in endometriosis through regulating Notch pathway"
作者全名："Dai, Xue-Shan; Wei, Qing-Hua; Guo, Xin; Ding, Yi; Yang, Xiao-Qian; Zhang, Yu-Xin; Xu, Xiao-Yu; Li, Cong; Chen, Yi"
作者地址："[Dai, Xue-Shan; Wei, Qing-Hua; Guo, Xin; Ding, Yi; Yang, Xiao-Qian; Zhang, Yu-Xin; Xu, Xiao-Yu; Chen, Yi] Southwest Univ, Coll Pharmaceut Sci & Chinese Med, Chongqing, Peoples R China; [Dai, Xue-Shan; Wei, Qing-Hua; Guo, Xin; Ding, Yi; Yang, Xiao-Qian; Zhang, Yu-Xin; Xu, Xiao-Yu; Chen, Yi] Chongqing Key Lab New Drug Screening Tradit Chines, Chongqing, Peoples R China; [Dai, Xue-Shan; Wei, Qing-Hua; Guo, Xin; Ding, Yi; Yang, Xiao-Qian; Zhang, Yu-Xin; Xu, Xiao-Yu; Chen, Yi] Southwest Univ, Coll Pharmaceut Sci & Chinese Med, Minist Educ, Engn Res Ctr Coptis Dev & Utilizat, Chongqing, Peoples R China; [Dai, Xue-Shan; Wei, Qing-Hua; Guo, Xin; Ding, Yi; Zhang, Yu-Xin; Xu, Xiao-Yu; Chen, Yi] Southwest Univ, Natl Demonstrat Ctr Expt Pharm Educ, Chongqing, Peoples R China; [Li, Cong] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing 400016, Peoples R China; [Chen, Yi] Southwest Univ, Coll Pharmaceut Sci & Chinese Med, 2 Tiansheng Rd, Chongqing 400715, Peoples R China"
通信作者："Chen, Y (通讯作者)，Southwest Univ, Coll Pharmaceut Sci & Chinese Med, 2 Tiansheng Rd, Chongqing 400715, Peoples R China."
来源：LIFE SCIENCES
ESI学科分类：BIOLOGY & BIOCHEMISTRY
WOS号：WOS:001046369500001
JCR分区：Q1
影响因子：5.2
年份：2023
卷号：328
期号：
开始页：
结束页：
文献类型：Article
关键词：Notch pathway; Proliferation; Ferulic acid; Ligustrazine; Tetrahydropalmatine; Endometriosis
摘要："Aims: With an ambiguous anti-proliferative mechanism, the combination of ferulic acid, ligustrazine, and tetrahydropalmatine (FLT) shows good anti-endometriosis (EMS) activity. In EMS, the expression of Notch pathway and its role in proliferation are not yet unclear. In this study, we sought to uncover the role of Notch pathway's effect and FLT's anti-proliferative mechanism on EMS proliferation.Main methods: In autograft and allograft EMS models, the proliferating markers (Ki67, PCNA), Notch pathway, and the effect of FLT on them were detected. Then, the anti-proliferative influence of FLT was measured in vitro. The proliferating ability of endometrial cells was investigated with a Notch pathway activator (Jagged 1 or VPA) or inhibitor (DAPT) alone, or in combination with FLT separately.Key findings: FLT presented the inhibitory effect on ectopic lesions in 2 EMS models. The proliferating markers and Notch pathway were promoted in ectopic endometrium, but FLT showed the counteraction. Meantime, FLT restrained the endometrial cell growth and clone formation along with a reduction in Ki67 and PCNA. Jagged 1 and VPA stimulated the proliferation. On the contrary, DAPT displayed the anti-proliferating effect. Furthermore, FLT exhibited an antagonistic effect on Jagged 1 and VPA by downregulating Notch pathway and restraining proliferation. FLT also displayed a synergistic effect on DAPT.Significance: This study indicated that the overexpressing Notch pathway induced EMS proliferation. FLT attenuated the proliferation by inhibiting Notch pathway."
基金机构："National Natural Science Foundation of China [81773984, 81402441]; Chongqing Natural Sci-ence Foundation of China [CSTC2020JCYJ-MSXMX0451]; Traditional Chinese medicine research project of Chongqing Health Bureau [2020ZY023665]; Chinese Medicine Rehabilitation - the Key Discipline Constructed by Chongqing Health Bureau [2021-4322190044]; Fundamental Research Funds for the Central Universities [XDJK2012C054]; Southwest University Doctor Foundation [SWU111074]"
基金资助正文："This work was supported by National Natural Science Foundation of China [grant numbers 81773984, 81402441] ; Chongqing Natural Sci-ence Foundation of China [grant numbers CSTC2020JCYJ-MSXMX0451] ; Traditional Chinese medicine research project of Chongqing Health Bureau [grant number 2020ZY023665] ; Chinese Medicine Rehabilitation - the Key Discipline Constructed by Chongqing Health Bureau [grant number 2021-4322190044] ; Fundamental Research Funds for the Central Universities [grant number XDJK2012C054] ; and Southwest University Doctor Foundation [grant number SWU111074] ."
