The effects of Gli1 and Gli2 on BMP9-induced osteogenic differentiation of mesenchymal stem cells

作者全名："Xu, Li; Ji, Caixia; Yu, Tingting; Luo, Jinyong"

作者地址："[Xu, Li] Third Hosp Mianyang, Sichuan Mental Hlth Ctr, Dept Clin Lab, Mianyang 621000, Sichuan, Peoples R China; [Ji, Caixia; Yu, Tingting; Luo, Jinyong] Chongqing Med Univ, Key Lab Diagnost Med, Chinese Minist Educ, Chongqing 400016, Peoples R China; [Luo, Jinyong] Chongqing Med Univ, Key Lab Diagnost Med, Chinese Minist Educ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Luo, JY (通讯作者)，Chongqing Med Univ, Key Lab Diagnost Med, Chinese Minist Educ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：TISSUE & CELL

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001046638600001

JCR分区：Q1

影响因子：2.7

年份：2023

卷号：84

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Hedgehog; BMP9; Mesenchymal stem cells; Signal Transduction

摘要："Diseases, such as bone nonunion with bone defects, osteoporosis, etc, seriously endanger people's quality of life, and bone tissue engineering based on mesenchymal stem cells is an effective method to solve such problems. Several studies have shown that BMP9 can effectively promote osteogenic differentiation of MSCs, but the underlying molecular mechanisms are still unclear. Gli1 and Gli2 were important transcription factors and play an important role in the Hedgehog signaling pathway. In this study, we investigated the role of Gli1 and Gli2 in BMP9-induced osteogenic differentiation of MSCs. We found that inhibition of Gli1 and Gli2 weakened BMP9induced osteogenic differentiation of MSCs, and early osteogenic markers (alkaline phosphatase, ALP), late osteogenic markers (calcium salt deposition), the expression of pivotal osteogenic markers were attenuated, and inhibition of Gli1 and Gli2 weakened the expression of p-Smad1/5/8 and p-p38 induced by BMP9. In conclusion, our study shows that Gli1 and Gli2 play an important role in BMP9-induced osteogenic differentiation."

基金机构：National Natural Science Foundation of China [81874001]

基金资助正文：We thank Dr Tong-Chuan He (University of Chicago Medical Center) for providing the recombinant adenoviruses. This study was supported in part by National Natural Science Foundation of China (Grant No.81874001) to Jinyong Luo.