Temporal and spatial effects on C-reactive protein's regulation of inducible nitric oxide synthase production in periodontal disease

作者全名："Li, Lingjie; Jia, Lurong; Hou, Siyu; Zhang, Tingwei; Zhou, Mengjiao; Chen, Tao; Song, Jinlin"

作者地址："[Li, Lingjie; Jia, Lurong; Hou, Siyu; Zhang, Tingwei; Zhou, Mengjiao; Chen, Tao; Song, Jinlin] Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China; [Li, Lingjie; Hou, Siyu; Zhang, Tingwei; Zhou, Mengjiao; Song, Jinlin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Jia, Lurong; Chen, Tao] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Song, JL (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China."

来源：JOURNAL OF PERIODONTOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001046838500001

JCR分区：Q1

影响因子：4.2

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：autophagy; C-reactive protein; inducible nitric oxide synthase; periodontal disease

摘要："BackgroundInducible nitric oxide synthase (iNOS) is associated with inflammation and osteoclastic differentiation in periodontal disease. This study was conducted to compare the time-dependent variation in iNOS production between the gingiva and other periodontal tissues and to explore the potential association with C-reactive protein (CRP) in early periodontal disease. MethodsLigature-induced periodontal disease models (0-14 days) were established in wild-type and CRP knockout rats. Changes in CRP, iNOS, and autophagy levels were examined in the gingiva and other periodontal tissues. Macrophages were treated with lipopolysaccharide and chloroquine to explore the role of autophagy in iNOS production. iNOS, CRP, and autophagy-related proteins were analyzed using Western blotting, immunostaining, and enzyme-linked immunosorbent assays. mRNA expression was detected by quantitative real-time polymerase chain reaction. Hematoxylin and eosin staining was used for histological analysis. Cathepsin K immunostaining and microcomputed tomography of the maxillae were performed to compare alveolar bone resorption. ResultsiNOS and CRP levels increased rapidly in periodontal tissues, as observed on Day 2 of ligature, then decreased more rapidly in the gingiva than in other periodontal tissues. CRP deficiency did not prevent iNOS generation, but effectively accelerated iNOS reduction and delayed alveolar bone loss. The CRP effect on iNOS was accompanied by a change in autophagy, which was reduced by CRP knockout. ConclusionsThe regulation of iNOS by CRP shows temporospatial variation in early periodontal disease and is potentially associated with autophagy. These findings may contribute to the early detection and targeted treatment of periodontal disease."

基金机构："National Natural Science Foundation of China [82001081, 31971282, 82170968]; Chongqing Postdoctoral Science Foundation [csts2020jcyj-bshX0022]; China Postdoctoral Science Foundation [2020M683266, dstd201903]; Post-doctoral Innovative Talent Support Program of Chongqing [2010010006080066]"

基金资助正文："National Natural Science Foundation of China, Grant/Award Numbers: 82001081,31971282, 82170968; China Postdoctoral Science Foundation, Grant/Award Number: 2020M683266; Chongqing Postdoctoral Science Foundation, Grant/Award Number:csts2020jcyj-bshX0022; 2019 Chongqing Graduate Tutor Team Construction Project, Grant/Award Number:dstd201903; Post-doctoral Innovative Talent Support Program of Chongqing, Grant/Award Number: 2010010006080066"