"The GLI2/CDH6 axis enhances migration, invasion and mitochondrial fission of stomach adenocarcinoma cells"

作者全名："Liu, Guang-yi; Wang, Huan; Ran, Rui; Wang, Yi-cheng; Li, Yang"

作者地址："[Liu, Guang-yi; Ran, Rui; Wang, Yi-cheng; Li, Yang] Chongqing Med Univ, Affiliated Hosp 2, Dept Gastrointestinal Surg, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Wang, Huan] Chongqing Med Univ, Affiliated Hosp 2, Dept Hlth Management Ctr, Chongqing 400010, Peoples R China"

通信作者："Li, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Gastrointestinal Surg, 74 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001046856900001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：676

期号：　

开始页：182

结束页：189

文献类型：Article

关键词：GLI2; CDH6; STAD; Mitochondrial fission

摘要："It has been reported that cadherin 6 (CDH6) upregulation is associated with enhanced epithelial-to-mesenchymal transition (EMT) in several types of solid tumor cells. The current study aimed to explore the effect of CDH6 on the migration and invasion of stomach adenocarcinoma (STAD) cells, the transcription factors involved in CDH6 dysregulation and their effect on mitochondrial fission. Bioinformatics analysis was performed using data extracted from the Genotype-Tissue Expression Project, the Cancer Genome Atlas and Kaplan-Meier plotter. AGS and HGC27 cells were used to establish an in vitro STAD cell model. The results showed that higher CDH6 expression was associated with significantly shorter overall survival in patients with STAD. In addition, CDH6 overexpression promoted wound healing, enhanced the invasion ability of tumor cells and increased mito-chondrial fission. Glioma-associated oncogene family zinc finger 2 (GLI2) could bind to the CDH6 promoter and activate its transcription. Fluorescent labeling also showed that GLI2 overexpression promoted mitochondrial fission. However, CDH6 silencing significantly reduced mitochondrial fragmentation. Besides, GLI2 over-expression notably upregulated phosphorylated-focal adhesion kinase and dynamin-related protein 1. However, the above effects were largely abrogated by CDH6 knockdown. In conclusion, the present study suggested that the novel GLI2/CDH6 axis could enhance the migration, invasion and mitochondrial fission of STAD cells."

基金机构：Chongqing Municipal Health Commis-sion [2019jstg033]

基金资助正文：Funding statement This work was supported by Chongqing Municipal Health Commis-sion (2019jstg033) .