Resolvin D1 Attenuates Inflammation and Pelvic Pain Associated with EAP by Inhibiting Oxidative Stress and NLRP3 Inflammasome Activation via the Nrf2/HO-1 Pathway

作者全名："Zhang, Jiong; Chen, Juan; Jiang, Qing; Feng, Rui; Zhao, Xiaohu; Li, Haolin; Yang, Cheng; Hua, Xiaoliang"

作者地址："[Zhang, Jiong; Jiang, Qing; Hua, Xiaoliang] Chongqing Med Univ, Dept Urol, Affiliated Hosp 2, Chongqing, Peoples R China; [Zhang, Jiong; Feng, Rui; Zhao, Xiaohu; Li, Haolin; Yang, Cheng] Anhui Med Univ, Dept Urol, Affiliated Hosp 1, Hefei, Peoples R China; [Chen, Juan] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China"

通信作者："Yang, C (通讯作者)，Anhui Med Univ, Dept Urol, Affiliated Hosp 1, 218 Jixi Rd, Hefei 230022, Peoples R China.; Hua, XL (通讯作者)，Chongqing Med Univ, Dept Urol, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：JOURNAL OF INFLAMMATION RESEARCH

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001048174100001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：16

期号：　

开始页：3365

结束页：3379

文献类型：Article

关键词：chronic prostatitis and chronic pelvic pain syndrome; CP; CPPS; resolvin D1; inflammation; NLRP3 inflammasome; oxidative stress

摘要："Background: Resolvin D1 (RvD1), a member of the specialized pro-resolving lipid mediators family, has a potent anti-inflammatory effect and alleviates tissue damage. The purpose of the current research was to study the effect of RvD1 on CP/CPPS and the underlying mechanisms using a mouse model of experimental autoimmune prostatitis (EAP) mice. Materials and Methods: The EAP mouse model was successfully established, and was used to test the therapeutic effect of RvD1. Hematoxylin-eosin staining and dihydroethidium staining were used to evaluate the histological changes and oxidative stress levels of prostate tissues. Chronic pelvic pain was assessed by applying von Frey filaments to the lower abdomen. The superoxide dismutase enzyme and malondialdehyde levels were detected using enzyme-linked immunosorbent assay (ELISA). The levels of inflammationrelated cytokines, including IL-1 beta, IL-6, and TNF-alpha were detected by ELISA. Results: RvD1 treatment ameliorated prostatic inflammation and the pelvic pain of EAP mice. RvD1 treatment could inhibit activation of the NLRP3 inflammasome and oxidative stress. RvD1 treatment could activate Nrf2/HO-1 signaling in mice with EAP. Blockade of Nrf2/HO-1 signaling abolished the RvD1-mediated inhibition of oxidative stress, NLRP3 inflammasome activation and the anti-inflammatory effect of RvD1 in EAP. Conclusion: RvD1 treatment can reduce inflammatory cell infiltration in prostate tissue and attenuate pelvic pain associated with EAP by inhibiting oxidative stress and NLRP3 inflammasome activation via the Nrf2/HO-1 pathway. These results provide new insights that RvD1 has the potential as an effective agent in the treatment of EAP."

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