Association between vaginal microbiomes and neonatal septicemia in pregnant women with preterm premature rupture of membranes based on metagenome sequencing

作者全名："Yang, Yong; Liu, Zhaoming; Chen, Xiaoyan; Qin, Feng; Zhou, Wei"

作者地址："[Yang, Yong; Liu, Zhaoming; Chen, Xiaoyan; Qin, Feng; Zhou, Wei] Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Obstet & Gynecol, Women & Childrens Hosp, Chongqing, Peoples R China; [Zhou, Wei] Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Obstet & Gynecol, Women & Childrens Hosp, 120 Longshan Rd, Chongqing 400010, Peoples R China"

通信作者："Zhou, W (通讯作者)，Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Obstet & Gynecol, Women & Childrens Hosp, 120 Longshan Rd, Chongqing 400010, Peoples R China."

来源：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001048556300010

JCR分区：Q3

影响因子：1.7

年份：2023

卷号：15

期号：7

开始页：4544

结束页：+

文献类型：Article

关键词：Preterm premature rupture of membranes; neonatal septicemia; microbiomes; whole genome shotgun; metabolic pathways

摘要："Background: Preterm premature rupture of membranes (PPROM) is closely associated with pathogenic microbiomes in the female reproductive tract, and can lead to neonatal septicemia. The current study aimed to investigate potential pathogenic microbiomes associated with neonatal septicemia based on DNA metagenome sequencing. Methods: In this study, a total of 7 pregnant women with PPROM presenting neonatal septicemia (experimental group) and 3 pregnant women with normal newborns (control group) were enrolled. Vaginal secretions at admission and before parturition as well as placental tissues after parturition were collected for DNA metagenome sequencing using whole genome shotgun method on the Illumina NovaSeq/HiSeq platform. Raw data were processed by BioBakery workflow, and MetaPhlAn4 was implemented for qualitative and quantitative analyses of microbiome. Lactobacillus crispatus, Gardneralla vaginalis, Fannyhessea vaginae and Streptococcus suis were specifically detected from the experimental group. The two groups were compared using Student's t-tests. Results: The indexes of Chao1 (P=0.00028/P=0.00072), abundance-based coverage estimator (ACE, P=0.00059/P=0.00026), Shannon (P=0.036/P=0.0065) and Simpson (P=0.007/P=0.041) in the experimental group were increased at admission and before parturition as compared with the control group. Several microbiomes, such as Lactobacillus crispatus, Gardneralla vaginalis, Fannyhessea vaginae and Streptococcus suis, were specifically detected in the experimental group. Notably, Gardnerella vaginalis and Streptococcus gallolyticus were identified from the vaginal secretions and placenta tissues of women with neonatal septicemia. Moreover, nucleic acid synthesis and carbohydrate metabolism-related pathways were enriched in the experimental group. Conclusion: This study enhanced the current understanding of the mechanisms underlying pathogenic microbiomes in PPROM-induced neonatal septicemia. The trial registry number is ChiCTR2300070666 (URL: https://www.chictr.org.cn/showproj.html?proj=195648)."

基金机构：Chongqing Science and Health Joint Medical Research Project [2019GDRC013]; High-level Medical Reserved Personnel Training Project of Chongqing

基金资助正文：This research was supported by High-level Medical Reserved Personnel Training Project of Chongqing and Chongqing Science and Health Joint Medical Research Project (No. 2019GDRC013).