Increased serum α-tocopherol acetate mediated by gut microbiota ameliorates alveolar bone loss through the STAT3 signalling pathway in diabetic periodontitis

作者全名："Lu, Miao; Zhang, Yanan; Yuan, Xulei; Zhang, Yang; Zhou, Mengjiao; Zhang, Tingwei; Song, Jinlin"

作者地址："[Lu, Miao; Zhang, Yanan; Yuan, Xulei; Zhang, Yang; Zhou, Mengjiao; Zhang, Tingwei; Song, Jinlin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing, Peoples R China"

通信作者："Zhang, TW; Song, JL (通讯作者)，Chongqing Med Univ, Stomatol Hosp, 426 Songshibei Rd, Chongqing 401147, Peoples R China."

来源：JOURNAL OF CLINICAL PERIODONTOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001050536100001

JCR分区：Q1

影响因子：5.8

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：diabetes; gut microbiota; periodontitis; STAT3 pathway; alpha-tocopherol acetate

摘要："Aim: To evaluate whether and how gut microbiota-meditated metabolites regulate alveolar bone homeostasis in diabetic periodontitis (DP). Materials and Methods: Lactobacillus casei (L. casei) was employed as a positive modulator of gut microbiota in DP mice. The destruction of alveolar bone was evaluated. Untargeted metabolomics was conducted to screen out the pivotal metabolites. A co-housing experiment was conducted to determine the connection between the gut microbiota and alpha-tocopherol acetate (alpha-TA). alpha-TA was applied to DP mice to investigate its effect against alveolar bone loss. Human periodontal ligament cells (hPDLCs) and human gingival fibroblasts (HGFs) were extracted for the in vitro experiment. Transcriptomic analysis and immunohistochemistry were performed to detect the major affected signalling pathways. Results: Positive regulation of the gut microbiota significantly attenuated alveolar bone loss and increased the serum alpha-TA level. The alteration in gut microbiota composition could affect the serum alpha-T (the hydrolysates of alpha-TA) level. alpha-TA could alleviate alveolar bone destruction in DP mice and alpha-T exert beneficial effects on hPDLCs and HGFs. Mechanistically, the STAT3 signalling pathway was the pivotal pathway involved in the protective role of alpha-TA. Conclusions: The gut microbiota-alpha-TA-STAT3 axis plays an important role in the regulation of diabetic alveolar bone homeostasis."

基金机构："National Key Research and Development Program of China [2022YFC2504200]; National Natural Science Foundation of China [31971282, 82170968, U22A20314]; Chongqing Graduate Tutor Team Construction Project"

基金资助正文："ACKNOWLEDGEMENTS This work was supported by grants from the National Key Research and Development Program of China (Grant no. 2022YFC2504200), the National Natural Science Foundation of China (Grant nos. 31971282, 82170968, U22A20314) and the Chongqing Graduate Tutor Team Construction Project (Grant no. dstd201903)."