Transcriptomic analysis reveals the promotion of lymph node metastasis by Helicobacter pylori infection via upregulating chemokine (C-X-C motif) receptor 2 expression in gastric carcinoma

作者全名："Zha, Lang; Guo, Xiong; Liang, Xiaolong; Chen, Yuedong; Gan, Deyong; Li, Wenwen; Wang, Ziwei; Zhang, Hongyu"

作者地址："[Zha, Lang; Guo, Xiong; Liang, Xiaolong; Wang, Ziwei; Zhang, Hongyu] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing 400010, Peoples R China; [Chen, Yuedong; Gan, Deyong] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing 400010, Peoples R China; [Li, Wenwen] Chongqing Med Univ, Fac Basic Med, Dept Pathol, Chongqing 400016, Peoples R China"

通信作者："Zhang, HY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing 400010, Peoples R China."

来源：GENES & DISEASES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001052624500001

JCR分区：Q1

影响因子：6.9

年份：2023

卷号：10

期号：6

开始页：2614

结束页：2621

文献类型：Article

关键词：CXCR2; Gastric carcinoma; Helicobacter pylori; Invasion; Lymph node metastasis

摘要："Gastric carcinoma (GC) progression is mainly caused by local aggression and lymph node metastasis. However, some patients with early T-stage disease have lymph node metas-tasis, whereas some patients with late T-stage disease do not have lymph node metastasis, which indicates that invasion and metastasis are not always sequential in some GC patients. In the present study, the data of 101 GC cases were acquired from TCGA and divided into T-late-N-negative and T-early-N-positive groups according to pathological stages. A total of 338 genes were identified as differential genes between the T-late-N-negative and T-early-N-positive groups. GSEA showed that epithelial cell signaling in the Helicobacter pylori (HP) infection pathway was enriched in the T-early-N-positive group. MB staining indicated that the HP infection rate was 63% (39/62) in N-positive patients compared to 42% (16/38) in N -negative patients. To investigate the potential mechanism, we focused on the gene chemokine (C-X-C motif) receptor 2 (CXCR2), which was not only clustered in the gene set of epithelial cells signaling in the HP infection pathway but also significantly upregulated in T-early-N-pos-itive GC by the analysis of the different genes based on the TCGA dataset. A meta-analysis showed that CXCR2 expression was positively correlated with N-stage but not with T-stage in GC. This study indicated that invasion and metastasis could be independent processes driven by different molecular mechanisms in some GC patients. HP infection was a potential factor that promoted lymph node metastasis by upregulating CXCR2 expression.& COPY; 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons. org/licenses/by-nc-nd/4.0/)."

基金机构：National Natural Science Foundation of China; [81974385]

基金资助正文：<B>Funding</B> This work was supported by the National Natural Science Foundation of China (No. 81974385) .