IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4<SUP>+</SUP>T cells

作者全名："Zhang, Yanyu; Gao, Song; Yao, Shifei; Weng, Danlin; Wang, Yan; Huang, Qi; Zhang, Xuemei; Wang, Hong; Xu, Wenchun"

作者地址："[Zhang, Yanyu; Yao, Shifei; Weng, Danlin; Wang, Yan; Huang, Qi; Zhang, Xuemei; Wang, Hong; Xu, Wenchun] Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost Designated, Minist Educ, Chongqing, Peoples R China; [Gao, Song] Zunyi Med Univ, Sch Lab Med, Affiliated Hosp, Dept Lab Med, Zunyi, Guizhou, Peoples R China"

通信作者："Xu, WC (通讯作者)，Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost Designated, Minist Educ, Chongqing, Peoples R China."

来源：ISCIENCE

ESI学科分类：　

WOS号：WOS:001053398300001

JCR分区：Q1

影响因子：4.6

年份：2023

卷号：26

期号：8

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Vaccination is an effective means of preventing pneumococcal disease and SPY1 is a live attenuated pneumococcal vaccine we obtained earlier. We found IL-27 and its specific receptor (WSX-1) were increased in SPY1 vaccinated mice. Bacterial clearance and survival rates were decreased in SPY1 vaccinated IL-27R alpha(-/-) mice. The vaccine-induced Th17 cell response and IgA secretion were also suppressed in IL-27R alpha(-/-) mice. STAT3 and NF-kappa B signaling and expression of the Th17 cell polarization-related cytokines were also decreased in IL-27R alpha(-/-) bone-marrow-derived dendritic cells(BMDC) stimulated with inactivated SPY1. The numbers of memory CD4(+)T cells were also decreased in SPY1 vaccinated IL-27R alpha(-/-) mice. These results suggested that IL-27 plays a protective role in SPY1 vaccine by promoting Th17 polarization through STAT3 and NF-kappa B signaling pathways and memory CD4(+)T cells production in the SPY1 vaccine. In addition, we found that the immune protection of SPY1 vaccine was independent of aerobic glycolysis."

基金机构：National Natural Science Foundation of China [81671639]; [81960307]

基金资助正文："This study was supported by a grant from the National Natural Science Foundation of China (Grant No. 81960307 and Grant No. 81671639) . We thank the School of Laboratory Medicine, Chongqing Medical University, Chongqing, China; and all the authors involved in this study."