Maternal exposure to cetylpyridinium chloride impairs oogenesis by causing mitochondria disorder in neonates

作者全名："Liu, Ronglu; Mu, Xinyi; Gao, Rufei; Geng, Yanqing; Zhang, Yan; Chen, Xuemei; Yin, Xin; Wang, Hong; Li, Fangfang; He, Junlin"

作者地址："[Liu, Ronglu; Gao, Rufei; Zhang, Yan; Chen, Xuemei; Yin, Xin; Li, Fangfang; He, Junlin] Chongqing Med Univ, Sch Publ Hlth, Chongqing, Peoples R China; [Liu, Ronglu; Mu, Xinyi; Gao, Rufei; Geng, Yanqing; Zhang, Yan; Chen, Xuemei; Yin, Xin; Li, Fangfang; He, Junlin] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Chongqing, Peoples R China; [Geng, Yanqing] Chongqing Med Univ, Sch Basic Med, Chongqing, Peoples R China; [Wang, Hong] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China; [He, Junlin] Chongqing Med Univ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China"

通信作者："He, JL (通讯作者)，Chongqing Med Univ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001055869400001

JCR分区：Q1

影响因子：4.2

年份：2023

卷号：102

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Cetylpyridinium chloride; Quaternary ammonium compounds; Mitochondrial dysfunction; Neonatal ovary; MII oocyte

摘要："Cetylpyridinium Chloride (CPC) is a common disinfectant with potential mitochondrial toxicity. However, the effects of CPC on female reproduction remains unclear. In the present study, pregnant mice were exposed to environmentally relevant doses of CPC for 3 days, the effects were evaluated in the female offspring. Maternal exposure to CPC caused loss of oocytes in neonatal ovaries. TEM analysis of neonatal ovaries showed CPC caused aberrant mitochondrial morphology including vacuolated and disorganized structure, reduced functional cristae. In addition, CPC decreased mitochondrial membrane potential in neonatal oocytes. Seahorse analysis showed that CPC hampered mitochondrial reserve, manifested as reduced spare respiratory capacity. Furthermore, CPC damaged mitochondrial function and impaired developmental competence of MII oocytes, suggesting a persisting impact into adulthood. In summary, this is the first known demonstration that maternal exposure to CPC caused mitochondrial disorders in neonatal ovaries and had long-term effects on fertility of the female offspring."

基金机构：Special Professorship Project of Chongqing Medical University

基金资助正文："We are grateful for the financial support from the Special Professorship Project of Chongqing Medical University (2021-215) . Most of the materials in this manuscript were produced by the authors, and some image materials of the graphic abstract were obtained from the online free resource: biorender.com ."