Integration of gut microbiota and metabolomics for the hematopoiesis of Siwu paste on anemia rats

作者全名："Wan, Dan; Liang, Xuejuan; Yang, Limei; He, Dan; Du, Qing; Zhang, Wanping; Huang, Jianji; Xu, Linben; Cai, Ping; Huang, Jianhua; Xiong, Yiying; Zhou, Rongrong; Peng, Yongbo; Zhang, Shuihan"

作者地址："[Wan, Dan; Liang, Xuejuan; He, Dan; Du, Qing; Xu, Linben; Cai, Ping; Huang, Jianhua; Zhou, Rongrong; Zhang, Shuihan] Hunan Univ Chinese Med, Hunan Acad Chinese Med, Changsha 410013, Hunan, Peoples R China; [Wan, Dan; Liang, Xuejuan; He, Dan; Du, Qing; Xu, Linben; Cai, Ping; Huang, Jianhua; Zhang, Shuihan] Hunan Acad Chinese Med, Innovat Med Inst Tradit Chinese Med, Inst Chinese Med Resources, Changsha 410013, Hunan, Peoples R China; [Yang, Limei; Zhang, Wanping; Huang, Jianji; Xiong, Yiying; Peng, Yongbo] Chongqing Med Univ, Affiliated Hosp 1, Coll Pharm, Int Med Coll,Key Lab Biochem & Mol Pharmacol,Chong, Chongqing 400016, Peoples R China; [Zhou, Rongrong] Hunan Acad Chinese Med, Affiliated Hosp, Changsha 410013, Hunan, Peoples R China; [Zhou, Rongrong; Zhang, Shuihan] Hunan Univ Chinese Med, Changsha 410013, Hunan, Peoples R China; [Zhou, Rongrong; Zhang, Shuihan] Hunan Acad Chinese Med, Changsha 410013, Hunan, Peoples R China"

通信作者："Peng, YB (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Coll Pharm, Int Med Coll,Key Lab Biochem & Mol Pharmacol,Chong, Chongqing 400016, Peoples R China.; Zhou, RR; Zhang, SH (通讯作者)，Hunan Univ Chinese Med, Changsha 410013, Hunan, Peoples R China.; Zhou, RR; Zhang, SH (通讯作者)，Hunan Acad Chinese Med, Changsha 410013, Hunan, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001056376600001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：Siwu paste; Intestinal flora; Tryptophan metabolism; Anemia; Firmicutes/bacteroidetes

摘要："Background: To investigate the regulation mechanism of hematopoiesis of Siwu paste (SWP) in anemia rats, which is a classic Chinese prescription used for nourishing blood or blood deficiency over 1000 years.Methods: Blood cell and biochemical analysis were used to evaluate the hematopoietic function of SWP in anemia rats. The intestinal microbial composition was analyzed with 16S rRNA gene sequencing, and the metabolites were profiled using UPLC-TripleTOF system nontargeting metabolomics.Results: SWP can improve the levels of red blood cells, hemoglobin, platelet, hematocrit value, white blood cells, lymphocyte, EPO, TPO, and GM-CSF in anemia rats, and significantly change the microbial community and its metabolites. The correlation analysis of intestinal microbiota-hematopoietic efficacy shows that 13 kinds of different intestinal flora were related to hemato-poietic efficacy, in which Prevotella_1, Prevotella_9, Lactobacillus, and norank_f__Muribaculaceae were significantly positively correlated with hematopoiesis, nine kinds of intestinal flora are negatively correlated with hematopoietic effect. Compared with anemia rats, 218 potential metabolic biomarkers and 36 metabolites with significant differences were identified in the SWP treatment group, and the key metabolites were mainly amino acids and lipids. An in-depth analysis of metabolic pathways showed that SWP mainly affected 7 metabolic pathways, including aminobenzoic acid degradation and tryptophan metabolism.Conclusion: The study provides novel insights into the regulation of hematopoiesis of SWP in anemia rats that were correlated with gut microbiota and the metabolites, which through the restoration of the firmicutes/bacteroidetes ratio."

基金机构："Natural Science Foundation of Hunan Province [2021168]; Hunan Traditional Chinese Med- icine Research Project [A2022002, 2021NK4244]; Hunan Province Innovation Platform and Talent Plan [2019NK2101]; Key Research and Development Project of Hunan Province [CSTB2022NSCQ- MSX0857]; Natural Science Foundation of Chongqing; [2020JJ5328]"

基金资助正文："This work was supported by the Natural Science Foundation of Hunan Province (2020JJ5328) , Hunan Traditional Chinese Medicine Research Project (2021168, A2022002) , Hunan Province Innovation Platform and Talent Plan (No. 2021NK4244) , Key Research and Development Project of Hunan Province (2019NK2101) and the Natural Science Foundation of Chongqing (CSTB2022NSCQ- MSX0857) ."