Baricitinib improves pulmonary fibrosis in mice with rheumatoid arthritis-associated interstitial lung disease by inhibiting the Jak2/Stat3 signaling pathway

作者全名："Liu, Hongli; Yang, Yan; Zhang, Jie; Li, Xuelin"

作者地址："[Liu, Hongli; Yang, Yan] Med Univ, Dept Geriatr, Chongqing 400010, Peoples R China; [Zhang, Jie; Li, Xuelin] Chongqing Med Univ, Dept Geriatr, Chongqing 400010, Peoples R China"

通信作者："Zhang, J (通讯作者)，Chongqing Med Univ, Dept Geriatr, Chongqing 400010, Peoples R China."

来源：ADVANCES IN RHEUMATOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001056963900001

JCR分区：Q3

影响因子：2

年份：2023

卷号：63

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Rheumatoid arthritis; Interstitial lung disease; Baricitinib; Jak2/Stat3

摘要："Objective The study explored improvements in pulmonary inflammation and fibrosis in a bovine type II collagen-induced rheumatoid arthritis-associated interstitial lung disease mouse model after treatment with baricitinib and the possible mechanism of action. Methods A rheumatoid arthritis-associated interstitial lung disease mouse model was established, siRNA Jak2 and lentiviral vectors were transfected with human embryonic lung fibroblast cells. And the levels of relevant proteins in mouse lung tissue and human embryonic lung fibroblasts were detected by Western blotting. Results The levels of JAK2, p-JAK2, p-STAT3, p-SMAD3, SMA, TGF ss R2, FN and COL4 were increased in the lung tissues of model mice (P < 0.5) and decreased after baricitinib intervention (P < 0.05). The expression levels of p-STAT3, p-SMAD3, SMA, TGF ss R2, FN and COL4 were reduced after siRNA downregulation of the JAK2 gene (P < 0.01) and increased after lentiviral overexpression of the JAK2 gene (P < 0.01). Conclusion Baricitinib alleviated fibrosis in the lung tissue of rheumatoid arthritis-associated interstitial lung disease mice, and the mechanism of action may involve the downregulation of Smad3 expression via inhibition of the Jak2/Stat3 signaling pathway, with consequent inhibition of the profibrotic effect of transforming growth factor-ss 1."

基金机构：Not applicable.

基金资助正文：Not applicable.