Probiotic <i>Escherichia coli</i> Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice

作者全名："Shi, Jun; Ma, Dongxue; Gao, Shanhu; Long, Fei; Wang, Xin; Pu, Xingyu; Cannon, Richard D.; Han, Ting-Li"

作者地址："[Shi, Jun; Ma, Dongxue; Gao, Shanhu; Long, Fei; Wang, Xin; Pu, Xingyu; Han, Ting-Li] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing, Peoples R China; [Shi, Jun; Ma, Dongxue; Gao, Shanhu; Long, Fei; Wang, Xin; Pu, Xingyu; Han, Ting-Li] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing, Peoples R China; [Cannon, Richard D.] Univ Otago, Sir John Walsh Res Inst, Fac Dent, Dept Oral Sci, Dunedin, New Zealand; [Han, Ting-Li] Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynaecol, Chongqing, Peoples R China"

通信作者："Han, TL (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing, Peoples R China.; Han, TL (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing, Peoples R China.; Han, TL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynaecol, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001057245600001

JCR分区：Q2

影响因子：4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：probiotics; Escherichia coli Nissle 1917; outer membrane vesicles; diabetes; obesity; gut microbiota; metabolomics

摘要："Introduction: Obesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.Methods: In this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.Results: EcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of Firmicutes/Bacteroidetes in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ?-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.Discussion: Overall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes."

基金机构："This study was supported by the Foundation of State Key Laboratory of Ultrasound in Medicine and Engineering (2023KFKTOO2), Chongqing Science amp;amp; Technology Commission (cstc2021jcyj-msxmX0213), Chongqing Municipal Education Commission (KJZD-K20210040 [cstc2021jcyj-msxmX0213]; Foundation of State Key Laboratory of Ultrasound in Medicine and Engineering [KJZD-K202100407]; Chongqing Science amp;amp; Technology Commission [(2022) 15]; Chongqing Municipal Education Commission; Senior Medical Talents Program of Chongqing; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University; [2023KFKTOO2]"

基金资助正文："This study was supported by the Foundation of State Key Laboratory of Ultrasound in Medicine and Engineering (2023KFKTOO2), Chongqing Science & amp; Technology Commission (cstc2021jcyj-msxmX0213), Chongqing Municipal Education Commission (KJZD-K202100407), and Senior Medical Talents Program of Chongqing for Young and Middle-aged (2022) 15, and the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University."