LncRNA GAS5-hnRNPK axis inhibited ovarian cancer progression via inhibition of AKT signaling in ovarian cancer cells

作者全名："Zhang, Te; Leng, Yahui; Duan, Mengjing; Li, Zihang; Ma, Yongqing; Huang, Chengyang; Shi, Qin; Wang, Yi; Wang, Chengcheng; Liu, Dandan; Zhao, Xuan; Cheng, Shuang; Liu, Ao; Zhou, Yang; Liu, Jiaqi; Pan, Zhongqiu; Zhang, Huimei; Shen, Li; Zhao, Hongyan"

作者地址："[Zhang, Te; Leng, Yahui; Duan, Mengjing; Li, Zihang; Ma, Yongqing; Huang, Chengyang; Shi, Qin; Wang, Yi; Wang, Chengcheng; Liu, Dandan; Cheng, Shuang; Liu, Ao; Zhou, Yang; Liu, Jiaqi; Pan, Zhongqiu; Zhang, Huimei; Shen, Li; Zhao, Hongyan] Hubei Univ Med, Hubei Key Lab Embryon Stem Cell Res, Shiyan, Hubei, Peoples R China; [Zhao, Hongyan] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 3, Chongqing, Peoples R China; [Zhang, Te] Hubei Univ Med, Biomed Res Inst, Shiyan 442000, Hubei, Peoples R China; [Shen, Li] Hubei Univ Med, Taihe Hosp, Dept Clin Oncol, 30 South Renmin Rd, Shiyan 442000, Hubei, Peoples R China; [Duan, Mengjing] Hengdian Cent Hlth Ctr, Wuhan, Hubei, Peoples R China; [Zhao, Xuan] Xian Med Univ, Clin Coll 2, Xian, Shaanxi, Peoples R China"

通信作者："Shen, L; Zhao, HY (通讯作者)，Hubei Univ Med, Hubei Key Lab Embryon Stem Cell Res, Shiyan, Hubei, Peoples R China.; Zhao, HY (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 3, Chongqing, Peoples R China.; Shen, L (通讯作者)，Hubei Univ Med, Taihe Hosp, Dept Clin Oncol, 30 South Renmin Rd, Shiyan 442000, Hubei, Peoples R China."

来源：DISCOVER ONCOLOGY

ESI学科分类：　

WOS号：WOS:001057703200002

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：14

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Ovarian cancer; LncRNA; GAS5; hnRNPK

摘要："Background The incidence of ovarian cancer ranks third among gynecologic malignancies, but the mortality rate ranks first. Methods The expression of GAS5 is low in ovarian cancer and is associated with the low survival of ovarian cancer patients according to public ovarian cancer databases. GAS5 overexpression inhibited ovarian malignancy by affecting the proliferation and migratory abilities in OVCAR3 and A2780 cells. GAS5 overexpression increased the rate of cell apoptosis, and the cells were blocked in the G1 phase as assessed by flow cytometry. Results We found that hnRNPK was a potential target gene, which was regulated negatively by GAS5 based on RNApulldown and mass spectrometry analysis. Mechanistically, GAS5 affected the inhibition of the PI3K/AKT/mTOR pathways and bound the protein of hnRNPK, which influenced hnRNPK stability. Furthermore, rescue assays demonstrated hnRNPK was significantly involved in the progression of ovarian cancer. Conclusions Our study showed one of the mechanisms that GAS5 inhibited ovarian cancer metastasis by down-regulating hnRNPK expression, and GAS5 can be used to predict the prognosis of ovarian cancer patients."

基金机构：National Natural Science Foundation of China [32001024]; 67th batch China Postdoctoral Science Foundation [2020M673560XB]; Postdoctoral Natural Science Foundation of Chongqing Science and Technology Bureau [cstc2019jcyj-bsh0069]; Cultivating Project for Young Scholar at Hubei University of Medicine [2017QDJZR12]; Guiding Project of Hubei Provincial Health Committee [WJ2019F065]; Key Program of Hubei Province Education Department Fund [D20222101]; Major Program of Hubei Provincial Science Technology Department Fund [2022BCE012]; Guiding Project of Shiyan Science and Technology Bureau [22Y19]; Innovative Research Program for Graduates of Hubei University of Medicine [JC2022016]; Biomedical Research Institute

基金资助正文："This research was supported by the National Natural Science Foundation of China (32001024), The 67th batch China Postdoctoral Science Foundation (2020M673560XB), Postdoctoral Natural Science Foundation of Chongqing Science and Technology Bureau (cstc2019jcyj-bsh0069), Cultivating Project for Young Scholar at Hubei University of Medicine (2017QDJZR12), Guiding Project of Hubei Provincial Health Committee (WJ2019F065), Key Program of Hubei Province Education Department Fund (D20222101), Major Program of Hubei Provincial Science Technology Department Fund (2022BCE012), Guiding Project of Shiyan Science and Technology Bureau (22Y19). Innovative Research Program for Graduates of Hubei University of Medicine (JC2022016). The authors acknowledge the support of Biomedical Research Institute."