Clostridium butyricum MIYAIRI 588 alleviates periodontal bone loss in mice with diabetes mellitus

作者全名："Zhang, Yanan; Lu, Miao; Zhang, Yang; Yuan, Xulei; Zhou, Mengjiao; Xu, Xiaohui; Zhang, Tingwei; Song, Jinlin"

作者地址："[Zhang, Yanan; Lu, Miao; Zhang, Yang; Yuan, Xulei; Zhou, Mengjiao; Xu, Xiaohui; Zhang, Tingwei; Song, Jinlin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Stomatol Hosp, Chongqing, Peoples R China; [Zhang, Tingwei; Song, Jinlin] Chongqing Med Univ, Stomatol Hosp, 426 Songshibei Rd, Chongqing 401147, Peoples R China"

通信作者："Zhang, TW; Song, JL (通讯作者)，Chongqing Med Univ, Stomatol Hosp, 426 Songshibei Rd, Chongqing 401147, Peoples R China."

来源：ANNALS OF THE NEW YORK ACADEMY OF SCIENCES

ESI学科分类：Multidisciplinary

WOS号：WOS:001059881400001

JCR分区：Q1

影响因子：4.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：4-hydroxybenzenemethanol; Clostridium butyricum MIYAIRI 588; diabetes mellitus; gut microbiota; periodontitis

摘要："The gut microbiota is a bridge linking periodontitis and systemic diseases, such as diabetes mellitus (DM). The probiotic Clostridium butyricum MIYAIRI 588 (CBM588) is reportedly an effective therapeutic approach for gut dysbiosis. Here, in a mouse model, we explored the therapeutic effect of CBM588 on periodontal bone destruction in DM and DM-associated periodontitis (DMP), as well as the underlying mechanism. Micro-computed tomography revealed that DM and DMP both aggravated periodontal bone destruction, which was alleviated by intragastric supplementation with CBM588. Moreover, 16S rRNA sequencing and untargeted metabolite analysis indicated that CBM588 ameliorated DMP-triggered dysbiosis and led to reduced oxidative stress associated with elevated 4-hydroxybenzenemethanol (4-HBA) in serum. Furthermore, in vitro and in vivo experiments found that the metabolite 4-HBA promoted nuclear factor erythroid 2-related factor 2 (Nrf2) signaling activation and modulated the polarization of macrophages, thus ameliorating inflammatory bone destruction in DMP. Our study demonstrates the protective effects of CBM588 in DM-induced mice, with and without ligature-induced periodontitis. The mechanism involves regulation of the gut microbiota and restoration of the integrity of the gut barrier to alleviate oxidative damage by elevating serum 4-HBA. This study suggests the possibility of CBM588 as a therapeutic adjuvant for periodontal treatment in diabetes patients."

基金机构："National Key Research and Development Programof China [2022YFC2504200]; National Natural Science Foundation of China [82170968, U22A20314]; Chongqing Young and Middle-Aged Medical Excellence Team"

基金资助正文："National Key Research and Development Programof China, Grant/Award Number: 2022YFC2504200; National Natural Science Foundation of China, Grant/Award Numbers: 82170968, U22A20314; Chongqing Young and Middle-Aged Medical Excellence Team"