An all-in-one enzymatic DNA network based on catalytic hairpin assembly for label-free and highly sensitive detection of APE1

作者全名："Zhou, Shaoying; Li, Xingrong; Shu, Xiaojia; Cai, Xiaoying; Wu, Haiping; Ding, Shijia; Yan, Yurong"

作者地址："[Zhou, Shaoying; Shu, Xiaojia; Cai, Xiaoying; Wu, Haiping; Ding, Shijia; Yan, Yurong] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Zhou, Shaoying] Peoples Hosp Yubei Dist Chongqing City, Dept Clin Lab, Chongqing 401120, Peoples R China; [Li, Xingrong] Wuhan Univ, Dept Lab Med, Zhongnan Hosp, Wuhan 430071, Peoples R China"

通信作者："Zhou, SY; Yan, YR (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.; Zhou, SY (通讯作者)，Peoples Hosp Yubei Dist Chongqing City, Dept Clin Lab, Chongqing 401120, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001060420400001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：1278

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：APE1; Catalytic hairpin assembly; Enzymatic DNA network; All-in-one; Label-free detection

摘要："Apurinic/apyrimidinic endonuclease 1 (APE1), identified as a prospective cancer biomarker, plays a vital role in the occurrence and progression of cancer cell lines and impacts on genome stability. However, conventional approaches typically rely on the interactions between the antigen and antibody, limiting their utility for qualitative assessments of APE1 expression. Herein, an all-in-one enzymatic DNA network (EDN) assay with catalytic hairpin assembly for label-free and ultrasensitive detection of APE1 has been developed. In this work, the blocking strand can inhibit the initiator by obstructing the complementary region, preventing the hairpin from hybridizing in the absence of APE1 targets. While the presence of targets can activate the unlocking of the initiator, which can trigger the catalytic hairpin reaction, and increase the fluorescent signal. Under optimal conditions, the developed sensing method can detect the target APE1 down to 4.78 x 10-6 U mL-1 with a wide linear range from 5 x 10-6 U mL-1 to 30 U mL-1. This strategy has also been successfully applied to the analysis of complicated biological samples compared to ELISA, demonstrating its potential applications in biochemical and molecular biology research as well as clinical diagnostics. Overall, benefiting from the high amplification"

基金机构："National Natural Science Foundation of China [2018ZX10732202]; National Sci- ence and Technology Major Project of the Ministry of Science and Technology of China [cstc2018jcyjAX0349]; Natural Science Foundation Project of Chongqing; [81371904,81873980]"

基金资助正文："This work was funded by fi nancial support from the National Natural Science Foundation of China (81371904,81873980) , the National Sci- ence and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202) , and the Natural Science Foundation Project of Chongqing (cstc2018jcyjAX0349) ."