Expression of proline-rich protein 15 in breast cancer and its effect on cell biological function

作者全名："Tian, Jiao; Zhang, Yingzi; Qu, Chi; Li, Han; Liu, Shengchun"

作者地址："[Tian, Jiao; Zhang, Yingzi; Qu, Chi; Li, Han; Liu, Shengchun] Chongqing Med Univ, Affiliated Hosp 1, Dept Endocrine & Breast Surg, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Liu, SC (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Endocrine & Breast Surg, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：MACROMOLECULAR RESEARCH

ESI学科分类：CHEMISTRY

WOS号：WOS:001060916800001

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Breast cancer; PRR15; Cell Biology; Bioinformatics; Molecular cloning

摘要："PRR15, a member of the proline-rich protein family, has been linked to a better prognosis in breast cancer patients and is highly expressed in a variety of cancer tissues. However, further research must be done on its biological role in breast cancer and its expression. The purpose of this work is to investigate the role of PRR15 in the proliferation, migration, and apoptosis of breast cancer cells as well as to confirm the differential expression of PRR15 in various subtypes of breast cancer tissues and cells. To analyse the differential expression of PRR15 in paracancerous tissues and various types of breast cancer tissues, GEPIA, Kaplan-Meier plotter, GEO, TCGA, and other bioinformatics websites and public databases were used. PRR15 expression in breast cancer tissue was significantly higher than that in normal breast tissue, and PRR15 expression in ER + breast cancer was significantly higher than that in ER- breast cancer. The results were confirmed by RT-PCR, Western Using molecular cloning, plasmids were created to produce cells that over express PRR15. Experiments on how cells behave showed that PRR15 overexpression had no impact on the growth, migration, or death of ER+ and ER- breast cancer cells when compared to control cells. Furthermore, the biological behavior of ER + breast cancer cells was unaffected by PRR15 knockdown.Graphical abstractSchematic Representation PRR15 expression as unique biomarker for identification of cancer expression. When compared to control cells, experiments on how cells behave revealed that PRR15 overexpression had no effect on the proliferation, migration, or death of ER+ and ER- breast cancer cells. Furthermore, PRR15 knockdown had little impact on the biological behaviour of ER+ breast cancer cells"

基金机构：Natural Science Foundation of China for funding the current research.; Natural Science Foundation of China

基金资助正文：Natural Science Foundation of China for funding the current research.