Identification and Analysis of Neutrophil Extracellular Trap-Related Genes in Osteoarthritis by Bioinformatics and Experimental Verification

作者全名："Luan, Tiankuo; Yang, Xian; Kuang, Ge; Wang, Ting; He, Jiaming; Liu, Zhibo; Gong, Xia; Wan, Jingyuan; Li, Ke"

作者地址："[Luan, Tiankuo; Kuang, Ge; He, Jiaming; Gong, Xia] Chongqing Med Univ, Dept Anat, Chongqing, Peoples R China; [Yang, Xian; Wan, Jingyuan] Chongqing Med Univ, Dept Pharmacol, Chongqing, Peoples R China; [Wang, Ting; Liu, Zhibo] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 2, Chongqing, Peoples R China; [Li, Ke] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Wan, JY (通讯作者)，Chongqing Med Univ, Dept Pharmacol, Chongqing, Peoples R China.; Li, K (通讯作者)，Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：JOURNAL OF INFLAMMATION RESEARCH

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001061214900001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：16

期号：　

开始页：3837

结束页：3852

文献类型：Article

关键词：neutrophil extracellular traps; osteoarthritis; immune infiltration; TLR7; machine learning algorithms

摘要："Background: Osteoarthritis (OA) is a common joint disease with long-term pain and dysfunction that negatively affects the quality of life of patients. Neutrophil extracellular traps (NETs), consisting of DNA, proteins and cytoplasm, are released by neutrophils and play an important role in a variety of diseases. However, the relationship between OA and NETs is unclear. Methods: In our study, we used bioinformatics to explore the relationship between OA and NETs and the potential biological markers. GSE55235, GSE55457, GSE117999 and GSE98918 were downloaded from the Gene Expression Omnibus (GEO) database for subsequent analysis.After differential analysis of OA expression matrices, intersection with NET-related genes (NRGs) was taken to identify Differentially expressed NRGs (DE-NRGs) in OA processes. Evaluation of immune cell infiltration by ssGSEA and CIBERSORT algorithm. The GSVA method was used to analyze the activity changes of Neutrophils pathway, Neutrophil degranulation and Neutrophil granule constituents pathway. Results: Based on RandomForest (RF), Least Absolute Shrinkage and Selection Operator (LASSO), and Support Vector MachineRecursive Feature Elimination (SVM-RFE) learning algorithms, five core genes (CRISPLD2, IL1B, SLC25A37, MMP9, and TLR7) were identified to construct an OA-related nomogram model for predicting OA progression. ROC curve results for these genes validated the nomogram's reliability. Correlation analysis, functional enrichment, and drug predictions were performed for the core genes. TLR7 emerged as a key focus due to its high importance ranking in RF and SVM-RFE analyses. Gene Set Enrichment Analysis (GSEA) revealed a strong association between TLR7 and the Neutrophil extracellular trap pathway. Expression of core genes was demonstrated in mice OA models and human OA samples. TLR7 expression in ATDC5 cell line was significantly higher than control after TNF & alpha; induction, along with increased IL6 and MMP13. Conclusion: TLR7 may be related to NETs and affects OA."

基金机构：National Natural Science Foundation of China [81902293]

基金资助正文：This work was supported by the National Natural Science Foundation of China No.81902293(Ke. Li).