Integrative analysis reveals that SLC38A1 promotes hepatocellular carcinoma development via PI3K/AKT/mTOR signaling via glutamine mediated energy metabolism

作者全名："Feng, Hua-guo; Wu, Chuan-xin; Zhong, Guo-chao; Gong, Jian-ping; Miao, Chun-mu; Xiong, Bin"

作者地址："[Feng, Hua-guo; Wu, Chuan-xin; Zhong, Guo-chao; Gong, Jian-ping; Miao, Chun-mu; Xiong, Bin] Chongqing Med Univ, Dept Hepatobiliary Surg, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing, Peoples R China"

通信作者："Xiong, B (通讯作者)，Chongqing Med Univ, Dept Hepatobiliary Surg, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing, Peoples R China."

来源：JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001062847400003

JCR分区：Q3

影响因子：2.7

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：Hepatocellular carcinoma; Biomarker; PI3K/AKT/mTOR signaling; Glutamine; Metabolism-related genes

摘要："Although hepatocellular carcinoma (HCC) is rather frequent, little is known about the molecular pathways underlying its development, progression, and prognosis. In the current study, we comprehensively analyzed the deferentially expressed metabolism-related genes (MRGs) in HCC based on TCGA datasets attempting to discover the potentially prognostic genes in HCC. The up-regulated MRGs were further subjected to analyze their prognostic values and protein expressions. Twenty-seven genes were identified because their high expressions were significant in OS, PFS, DFS, DSS, and HCC tumor samples. They were then used for GO, KEGG, methylation, genetics changes, immune infiltration analyses. Moreover, we established a prognostic model in HCC using univariate assays and LASSO regression based on these MRGs. Additionally, we also found that SLC38A1, an amino acid metabolism closely related transporter, was a potential prognostic gene in HCC, and its function in HCC was further studied using experiments. We found that the knockdown of SLC38A1 notably suppressed the growth and migration of HCC cells. Further studies revealed that SLC38A1 modulated the development of HCC cells by regulating PI3K/AKT/mTOR signaling via glutamine mediated energy metabolism. In conclusion, this study identified the potentially prognostic MRGs in HCC and uncovered that SLC38A1 regulated HCC development and progression by regulating PI3K/AKT/mTOR signaling via glutamine mediated energy metabolism, which might provide a novel marker and potential therapeutic target in HCC."

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