Spherical nucleic acid enzyme programmed network to accelerate CRISPR assays for electrochemiluminescence biosensing applications

作者全名："Li, Yi; Liu, Mei-Ling; Liang, Wen-Bin; Zhuo, Ying; He, Xiao-Jing"

作者地址："[Li, Yi; Liu, Mei-Ling; He, Xiao-Jing] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Liang, Wen-Bin; Zhuo, Ying] Southwest Univ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing, Peoples R China"

通信作者："He, XJ (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:001063148900001

JCR分区：Q1

影响因子：10.7

年份：2023

卷号：238

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：CRISPR-Cas12a; Spherical nucleic acid enzyme; AuAg nanoclusters; Electrochemiluminescence; Metal-organic frameworks (MOFs)

摘要："Given the targeted binding ability and cleavage activity of the emerging CRISPR/Cas12a assay which transduces the target into its cleavage activity exhibited broadly prospective applications in integrated sensing and actuating system. Here, we elaborated a universal approach to quickly activate CRISPR/Cas12a for low-abundance biomarker detection based on the amplification strategy of a target-induced spherical nucleic acid enzyme (SNAzyme) network that could accelerate the output of activators. Specifically, multifunctional Y-shaped probes and hairpin probes (HPs, which contained the specific sequence of the activators of CRISPR/Cas12a and the substrate chain of DNAzyme) were rationally designed to construct SNAzyme. Target recognition induced disassembly of the Y-shaped probes, which released DNAzyme strands to active DNAzyme and accompanied by SNAzyme self-assembly into SNAzyme network. Interestingly, compared with randomly dispersed SNAzyme, the reaction kinetics of the SNAzyme network enhanced 1.6 times in response to A-methyl acyl-CoA racemase (AMACR, a biomarker for prostate cancer), which was attributed to the promoted catalytic efficiency of DNAzyme by the confined SNAzyme network. Benefiting from these, the prepared biosensor based on electrochemiluminescence (ECL) platform by loading AuAg nanoclusters (AuAgNCs) into metal-organic framework-5 (MOF-5) exhibited satisfying detection performance for AMACR with a wide linear range (0.001 & mu;g/mL to 100 & mu;g/mL) and a low detection limit (1.0 x 10-4 & mu;g/mL, which exhibited significant potential in clinical diagnoses."

基金机构：General program of Chongqing Natural Science Foundation [cstc2019jcyj-msxmX0073]; Senior Medical Talents program of Chongqing for Young and Middle aged; Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University

基金资助正文："Financial support from the General program of Chongqing Natural Science Foundation (cstc2019jcyj-msxmX0073), Senior Medical Talents program of Chongqing for Young and Middle aged, and Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University are acknowledged."