The severity of NEC is ameliorated by prostaglandin E2 through regulating intestinal microcirculation

作者全名："Mo, Dandan; Deng, Chun; Chen, Bailin; Ding, Xionghui; Deng, Qin; Guo, Hongjie; Chen, Gongli; Ye, Cuilian; Guo, Chunbao"

作者地址："[Mo, Dandan; Deng, Chun] Chongqing Med Univ, Dept Pediat, Yongchuan Hosp, 439 Xuanhua Rd, Xuanhua 402160, Peoples R China; [Chen, Bailin] Chongqing Med Univ, Dept Gen Surg, Childrens Hosp, 20 Jinyu Ave, Chongqing 400014, Peoples R China; [Ding, Xionghui] Chongqing Med Univ, Dept Burn, Childrens Hosp, 20 Jinyu Ave, Chongqing 400014, Peoples R China; [Deng, Qin] Chongqing Med Univ, Dept Nutr, Childrens Hosp, 20 Jinyu Ave, Chongqing 400014, Peoples R China; [Guo, Hongjie] Chongqing Med Univ, Dept Anesthesiol, Childrens Hosp, 20 Jinyu Ave, Chongqing 400014, Peoples R China; [Chen, Gongli; Ye, Cuilian; Guo, Chunbao] Chongqing Med Univ, Dept Pediat Surg, Chongqing Hlth Ctr Women & Children, Women & Childrens Hosp, 120 Longshan Rd, Chongqing 401147, Peoples R China; [Chen, Gongli; Ye, Cuilian; Guo, Chunbao] Chongqing Med Univ, Dept Pediat, Childrens Hosp, 120 Longshan Rd, Chongqing 401147, Peoples R China; [Ye, Cuilian] Chongqing Univ Technol, Sch Pharm & Bioengn, 69 Hongguang Ave, Chongqing 400054, Peoples R China"

通信作者："Ye, CL; Guo, CB (通讯作者)，Chongqing Med Univ, Dept Pediat Surg, Chongqing Hlth Ctr Women & Children, Women & Childrens Hosp, 120 Longshan Rd, Chongqing 401147, Peoples R China.; Ye, CL; Guo, CB (通讯作者)，Chongqing Med Univ, Dept Pediat, Childrens Hosp, 120 Longshan Rd, Chongqing 401147, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001064718300010

JCR分区：Q1

影响因子：3.8

年份：2023

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Prostaglandin E2 (PGE2) is implicated in intestinal inflammation and intestinal blood flow regulation with a paradoxical effect on the pathogenesis of necrotizing enterocolitis (NEC), which is not yet well understood. In the current study, we found that PGE2, EP4, and COX-2 varied at different distances from the most damaged area in the terminal ileum obtained from human infants with NEC. PGE2 administration alleviated the phenotype of experimental NEC and the intestinal microvascular features in experimental NEC, but this phenomenon was inhibited by eNOS depletion, suggesting that PGE2 promoted intestinal microcirculatory perfusion through eNOS. Furthermore, PGE2 administration increased the VEGF content in MIMECs under TNF alpha stress and promoted MIMEC proliferation. This response to PGE2 was involved in eNOS phosphorylation and nitric oxide (NO) production and was blocked by the EP4 antagonist in vitro, suggesting that targeting the PGE2-EP4-eNOS axis might be a potential clinical and therapeutic strategy for NEC treatment. The study is reported in accordance with ARRIVE guidelines (https://arriveguidelines.org)."

基金机构："National Natural Science Foundation of China [30973440, 30770950]; Chongqing Natural Science Foundation (CSTC) [2008BA0021, cstc2012jjA0155]"

基金资助正文："The research was supported by the National Natural Science Foundation of China (No: 30973440, 30770950), and the key project of the Chongqing Natural Science Foundation (CSTC, 2008BA0021, cstc2012jjA0155)."