Exploration of Perturbed Liver Fibrosis-Related Factors and Collagen Type I in Animal Model of Non-Alcoholic Fatty Liver Disease

作者全名："Wang, Liyun; Liu, Kahua; Deng, Liang; Zhou, Guanyu; Qian, Wei; Xu, Keshu"

作者地址："[Wang, Liyun] Shandong First Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Jinan 250014, Peoples R China; [Wang, Liyun] Shandong Prov Qianfoshan Hosp, Jinan 250014, Peoples R China; [Liu, Kahua] Cent Hosp Qingdao City, Dept Gastroenterol, Qingdao 266011, Shandong, Peoples R China; [Deng, Liang] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Chongqing 400016, Peoples R China; [Zhou, Guanyu] Sichuan Prov Peoples Hosp, Dept Gastroenterol, Chengdu 610072, Sichuan, Peoples R China; [Qian, Wei; Xu, Keshu] Huazhong Univ Sci & Technol, Dept Gastroenterol, Tongji Med Coll, Union Hosp, Wuhan 430022, Peoples R China"

通信作者："Xu, KS (通讯作者)，Huazhong Univ Sci & Technol, Dept Gastroenterol, Tongji Med Coll, Union Hosp, Wuhan 430022, Peoples R China."

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001064864700001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Non-alcoholic fatty liver disease center dot TGF-ss 1 center dot TGF-ss 3 center dot alpha-SMA center dot Liver fibrosis

摘要："To determine their involvement in the onset of the disease, we investigated the changing levels of liver fibrosis-related proteins, namely, type-I collagen, alpha-smooth muscle actin (alpha-SMA), and transforming growth factor ss 1 and ss 3 (TGF-ss 1, ss 3). The four groups of Sprague-Dawley (SD) rats were involved in the study, namely, (i) normal control group, (ii) high-fat diet group (HFD), (iii) carbon tetrachloride ( CCl4) group, and (iv) NAFLD group (animal model) which were chosen at random. The NAFLD model received HFD combined with subcutaneous injection of small doses of CCl4. Histopathological examination confirmed extent of liver fibrosis, while other immunological and molecular methods were used to evaluate expression and distribution of alpha-SMA, type I collagen TGF-ss 1 and TGF ss 3, at both m-RNA and protein levels. In contrast to the normal control group, the NAFLD group showed moderately elevated expressions of TGF-ss 1, a-SMA, and type I collagen, which was proportional on temporal scale of NAFLD persistence in the model (P < 0.05). In the early phage of NAFLD, enhancement in the mRNA transcripts and, henceforth, protein expression of TGF-ss 3 was observed. However, these were found to be downregulated in case of liver fibrosis (P < 0.05). This NAFLD rat model shows the histopathologic changes of human NAFLD and is suitable for the study of NAFLD pathogenesis. These findings suggest that type I collagen and the liver fibrosis-related factors TGF- ss 1, TGF ss 3, and alpha-SMA may be significant contributors to NAFLD. Although NAFLD model is previously demonstrated by other researchers, our study is novel in terms of exploration of involvement of fibrosis-related factors and in particular aforementioned proteins at the early stage of NAFLD vis-a-vis dynamics of type-I collagen distribution."

基金机构："Key Research and Development Project of Shandong Province, China [2019GSF108132]"

基金资助正文："This work was sponsored by the Key Research and Development Project of Shandong Province, China (NO.2019GSF108132)"