Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma

作者全名："Wang, Ying; Zhao, Guan-An; Li, Xia; Zhang, En; Tan, Wei; Chen, Jia-qi"

作者地址："[Wang, Ying] Ningbo Univ, Affiliated Lihuili Hosp, Dept Pharm, Ningbo, Zhejiang, Peoples R China; [Zhao, Guan-An] Peoples Hosp Lishui, Urinary Surg, Lishui, Zhejiang, Peoples R China; [Li, Xia; Zhang, En] Ningbo Univ, Affiliated Lihuili Hosp, Clin Lab, Ningbo, Zhejiang, Peoples R China; [Tan, Wei] Chongqing Med Univ, Affiliated Hosp 3, Gener Hosp, Chongqing, Peoples R China; [Chen, Jia-qi] Peoples Hosp Lishui, Clin Lab, Lishui, Zhejiang, Peoples R China"

通信作者："Tan, W (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Gener Hosp, Chongqing, Peoples R China.; Chen, JQ (通讯作者)，Peoples Hosp Lishui, Clin Lab, Lishui, Zhejiang, Peoples R China."

来源：FRONTIERS IN PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001064904900001

JCR分区：Q1

影响因子：4.4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：UPLC-MS/MS; rat; safinamide; plasma; pharmacokinetics

摘要："A fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standard (IS). The analytes were separated on an Acquity UPLC C18 (2.1mm x 50 mm, 1.7 mu m) chromatographic column with gradient elution using a mobile phase (0.1% formic acid-acetonitrile). Then, the eluates were detected by electrospray ionization (ESI) in positive ion mode. The analytes were quantified by multiple reaction monitoring (MRM) using the transition m/z 303.3.215.0 of safinamide and m/z 285.0.154.0 of IS. Safinamide had good linearity in the concentration range of 1.0-2000 ng/mL, and the lower limit of quantitation (LLOQ) was 1.0 ng/mL. The intra- and inter-day precision and accuracy of safinamide were less than 7.63%, while the average recovery rate was 92.98%-100.29%. The method was validated to be stable and had low noise, short chromatographic run time, wide linear range, small sample volumes, low sample injection volumes, and high sensitivity. Therefore, it can be used in pharmacokinetics and preclinical and clinical studies."

基金机构：Public Welfare Technology Research Project of Zhejiang Province [LGC22H100002]

基金资助正文：This study was supported by grants from the Public Welfare Technology Research Project of Zhejiang Province (Grant No. LGC22H100002).