Prevalence and association of sIgA in saliva and <i>Pseudomonas aeruginosa</i> infection in TB patients: a cross-sectional study

作者全名："Wan, Keqiang; Su, Chang; Yin, Fang; Yao, Caoyuan"

作者地址："[Yao, Caoyuan] Chongqing Med Univ, Dept Resp & Crit Care Med, YongChuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China; [Wan, Keqiang; Su, Chang; Yin, Fang] Chongqing Med Univ, Dept Infect Dis, Yongchuan Hosp, Chongqing, Peoples R China"

通信作者："Yao, CY (通讯作者)，Chongqing Med Univ, Dept Resp & Crit Care Med, YongChuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China."

来源：TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001065096600001

JCR分区：Q4

影响因子：0.6

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：immunity; immunoglobulin A; lung infection; pathogenic; Pseudomonas aeruginosa; respiratory tract; saliva

摘要："Objectives: Pseudomonas aeruginosa is pathogenic in immunocompromised individuals. It has several complex mechanisms for evading human immunity. The objective of the study was to examine the secretory immunoglobulin A (sIgA) mediated immune response in saliva to detect P. aeruginosa in pulmonary tuberculosis. Methods: The infection with P. aeruginosa was categorized according to the Leeds criteria in the final 86 individuals who were proven to have pulmonary tuberculosis by polymerase chain reaction. Levels of serum immunoglobulin G (IgG) and sIgA which are specific to P. aeruginosa were measured using the method of ELISA. Results: Patients in the ""free of infection (patients who were infected with P. aeruginosa in the lower respiratory tract at the beginning of the study later became negative)"" and ""intermittent colonized (patients who were infected with P. aeruginosa throughout the study)"" groups had substantially higher median baseline sIgA levels in saliva and a much greater proportion of sIgA positive than patients who were never colonized (patients who were found to be P. aeruginosa negative throughout the study) (p=0.038). Median baseline IgG level was 10.7 (1.7-145.0), 8.3 (2.5- 22.9), and 6.7 (3.3-17.1) for the patients categorized as ""intermittent colonization"", ""free of infection"" and ""never colonized"", respectively. After 3 years of study, sIgA level was found in significant high level among the patients with infection of P. aeruginosa (p=0.003). Conclusions: Secretory IgA may be readily collected from saliva and is a useful diagnostic technique for determining whether P. aeruginosa infection has occurred."

基金机构："The authors are thankful for the medical and non-medical staff of the YongChuan Hospital of Chongqing Medical University, Chongqing, China.; Chongqing, China"

基金资助正文："The authors are thankful for the medical and non-medical staff of the YongChuan Hospital of Chongqing Medical University, Chongqing, China."