Human milk oligosaccharides in milk of mothers with term and preterm delivery at different lactation stage

作者全名："Huang, Chuncui; Lu, Yue; Kong, Lin; Guo, Zhendong; Zhao, Keli; Xiang, Zheng; Ma, Xinyue; Gao, Huanyu; Liu, Yongfang; Gao, Zhongmin; Xu, Lijuan; Chai, Wengang; Li, Yan; Zhao, Yao"

作者地址："[Huang, Chuncui; Guo, Zhendong; Zhao, Keli; Ma, Xinyue; Gao, Huanyu; Li, Yan] Chinese Acad Sci, Inst Biophys, Key Lab Epigenet Regulat & Intervent, 15 Datun Rd, Beijing 100101, Peoples R China; [Lu, Yue; Kong, Lin; Xiang, Zheng; Liu, Yongfang; Gao, Zhongmin; Xu, Lijuan; Li, Yan; Zhao, Yao] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders,Childrens, Minist Educ,Key Lab Child Dev & Disorders, Chongqing Key Lab Child Infect & Immun,China Int S, Chongqing 400015, Peoples R China; [Guo, Zhendong; Ma, Xinyue; Li, Yan] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China; [Huang, Chuncui; Gao, Huanyu; Li, Yan] Western Inst Hlth Data Sci, 28 High Tech Ave, Chongqing 401329, Peoples R China; [Chai, Wengang] Imperial Coll London, Fac Med, Glycosciences Lab, London W12 0NN, England"

通信作者："Li, Y (通讯作者)，Chinese Acad Sci, Inst Biophys, Key Lab Epigenet Regulat & Intervent, 15 Datun Rd, Beijing 100101, Peoples R China.; Li, Y; Zhao, Y (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders,Childrens, Minist Educ,Key Lab Child Dev & Disorders, Chongqing Key Lab Child Infect & Immun,China Int S, Chongqing 400015, Peoples R China.; Li, Y (通讯作者)，Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China.; Li, Y (通讯作者)，Western Inst Hlth Data Sci, 28 High Tech Ave, Chongqing 401329, Peoples R China."

来源：CARBOHYDRATE POLYMERS

ESI学科分类：CHEMISTRY

WOS号：WOS:001065104200001

JCR分区：Q1

影响因子：10.7

年份：2023

卷号：321

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Human milk oligosaccharides (HMOs); Duration of gestation; Distinctive oligosaccharides; Preterm delivery; Phenotype

摘要："Human milk oligosaccharides (HMOs) are structurally diverse unconjugated glycans, and play crucial roles in protecting infants from infections. Preterm birth is one of the leading causes of neonatal mortality, and preterm infants are particularly vulnerable and are in need of improved outcomes from breast-feeding due to the presence of bioactive HMOs. However, studies on specific difference in HMOs as a function of gestation time have been very limited. We established an approach to extract and analyze HMOs based on 96-well plate extraction and mass spectrometry, and determined maternal phenotypes through distinctive fragments in product-ion spectra. We enrolled 85 women delivering at different gestation times (25-41 weeks), and observed different HMOs correlating with gestation time based on 233 samples from the 85 donors. With the increase of postpartum age, we observed a regular changing trajectory of HMOs in composition and relative abundance, and found significant differences in HMOs secreted at different postpartum times. Preterm delivery induced more variations between participants with different phenotypes compared with term delivery, and more HMOs varied with postpartum age in the population of secretors. The sialylation level in mature milk decreased for women delivering preterm while such decrease was not observed for women delivering on term."

基金机构："Biological Resources Programme, Chinese Academy of Sciences [2022YFC3400801]; National Key Research and Development Program of China [CSTB2023NSCQ-MSX0131]; National Natural Science Foundation of Chongqing, China [XDB38010200]; Strategic Priority Research Program of Chinese Academy of Sciences [22-FY18-821]; March of Dimes Prematurity Research Centre grant [218304/Z/19/Z]; Wellcome Trust Biomedical Resource grant; [KFJ-BRP-017-76]"

基金资助正文："This work was supported by Biological Resources Programme, Chinese Academy of Sciences (KFJ-BRP-017-76) and the National Key Research and Development Program of China (2022YFC3400801) . This work was also supported, in part, by the National Natural Science Foundation of Chongqing, China (CSTB2023NSCQ-MSX0131) and the Strategic Priority Research Program of Chinese Academy of Sciences (XDB38010200) , the March of Dimes Prematurity Research Centre grant (22-FY18-821) , and the Wellcome Trust Biomedical Resource grant (218304/Z/19/Z) . For the purpose of open access, the authors have applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission."