Anti-inflammatory effect of <i>Lactiplantibacillus plantarum</i> T1 cell-free supernatants through suppression of oxidative stress and NF-κB- and MAPK-signaling pathways

作者全名："Hao, Rui; Liu, Qianqian; Wang, Lu; Jian, Wenwen; Cheng, Yu; Zhang, Qiuyue; Hayer, Kim; Idris, Raja Kamarudin Raja; Zhang, Yi; Lu, He; Tu, Zeng"

作者地址："[Hao, Rui; Liu, Qianqian; Wang, Lu; Jian, Wenwen; Lu, He; Tu, Zeng] Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Dept Pathogen Biol, Chongqing, Peoples R China; [Hao, Rui] Hunan Univ Med, Sch Publ Hlth & Lab Med, Huaihua, Peoples R China; [Cheng, Yu; Zhang, Qiuyue; Zhang, Yi] Chongqing Med Univ, Int Med Coll, Chongqing, Peoples R China; [Hayer, Kim; Idris, Raja Kamarudin Raja] Univ Leicester, Leicester Med Sch, Leicester, England"

通信作者："Tu, Z (通讯作者)，Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Dept Pathogen Biol, Chongqing, Peoples R China."

来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001066363800001

JCR分区：Q2

影响因子：3.9

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Lactiplantibacillus plantarum; RAW264.7 macrophages; oxidative stress; NF-kappa B-signaling pathway; MAPK-signaling pathway

摘要："Lactiplantibacillus plantarum T1 is an isolated probiotic lactic acid bacterium (LAB) from pickled vegetables in Chongqing, China. In this study, we evaluated the anti-inflammatoryanti-inflammatory activity and the underlying mechanisms of L. plantarum T1 cell-free supernatant (CFS) on lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages in vitro. Reverse transcription quantitative PCR (RT-qPCR), immunofluorimmunofluorescence,escence, Griess methods, and western blotting were utilized to assess the anti-inflamanti-inflammatorymatory cytokines and antioxidative effecteffect of L. plantarum T1 CFS. Our results showed that L. plantarum T1 CFS pretreatment significantlysignificantly reduced pro-inflammatorypro-inflammatory cytokine levels, including nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor, interleukin (IL)-1 ss, and IL-6, as well as reactive oxygen species. Interestingly, L. plantarum T1 CFS unregulated the antioxidant indicators, including superoxide dismutase, catalase, and glutathione in RAW264.7 cells. Furthermore, L. plantarum T1 CFS activated the nuclear factor kappa-B (NF-kappa B) and mitogen-activated protein kinase (MAPK) pathway. This study showed the excellent antioxidant and anti-inflammatoryanti-inflammatoryanti-inflammatoryproperties of L. plantarum T1 through multiple pathways, highlighting its potential for further research and application as a probiotic strain. IMPORTANCE L. plantarum T1 stood out in a series of acid and bile salt tolerance and bacterial inhibition tests as a probiotic isolated from paocai, which provides many health benefits to the host by inhibiting the growth of harmful pathogenic microorganisms and suppressing excessive levels of oxidative stress and inflammation. Not all LAB have good probiotic functions and are used in various applications. The anti-inflammatoryanti-inflammatoryanti-inflammatoryantioxidant potential and mechanisms of L. plantarum T1 CFS have not been described and reported. By using RT-qPCR, Griess method, and western blotting, we showed that L. plantarum T1 CFS had anti-inflammatoryanti-inflammatory and antioxidant effects.effects. Griess assay, TBA assay, WST-8 assay, immunofluorescenceimmunofluorescence assay, RT-qPCR, and western blotting data revealed that its anti-inflammatoryanti-inflammatory and antioxidant mechanisms were associated with oxidative stress and NF-.B and MAPK signaling pathways. The anti-inflammatoryanti-inflammatory and antioxidant effectseffects of L. plantarum T1 CFS in paocai generates opportunities for probiotic product development."

基金机构：Natural Science Foundation of Chongqing [cstc2021jcyjmsxmX0158]; Education Teaching Reform Research Project of Chongqing [223115]; Education Teaching Research Project of Chongqing Medical University [JY210301]

基金资助正文：Natural Science Foundation of Chongqing no. cstc2021jcyjmsxmX0158 Zeng Tu; Education Teaching Reform Research Project of Chongqing 223115 Zeng Tu; Education Teaching Research Project of Chongqing Medical University JY210301 Zeng Tu