Overcoming Aggregation-Caused Quenching by an Improved Porphyrin Hybrid and Its Application in Enhanced Electrochemiluminescence Biosensing

作者全名："Wu, Yijie; Mu, Zhaode; Gong, Feng; Qing, Min; Zhou, Jing; Chen, Keyu; Bai, Lijuan"

作者地址："[Wu, Yijie; Chen, Keyu; Bai, Lijuan] Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Coll Pharm, Chongqing 400016, Peoples R China; [Mu, Zhaode; Qing, Min; Zhou, Jing] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongq, Chongqing 400016, Peoples R China; [Gong, Feng] Chongqing Ind & Informat Vocat Coll, Chongqing 400043, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Coll Pharm, Chongqing 400016, Peoples R China."

来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

ESI学科分类：CHEMISTRY

WOS号：WOS:001067264100001

JCR分区：Q1

影响因子：7.1

年份：2023

卷号：11

期号：38

开始页：14124

结束页：14132

文献类型：Article

关键词："electrochemiluminescence; non-small-cell lung cancer; biosensor; 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin; CYFRA21-1; aggregation-caused quenching"

摘要："Research in the field of electrochemiluminescence (ECL) has been centered on the hunt for efficient electrochemiluminescent materials but remains a great challenge. In this paper, we report a metalized porphyrin hybrid that exhibits high ECL emission efficiency and apply it as a new luminophore for the first time. A lanthanum metal functionalized organic ligand 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) hybrid (LPH) was synthesized by a one-pot solvothermal method. TCPP exhibits a relatively obvious aggregation-caused quenching (ACQ) effect in aqueous media, which is responsible for its undesirable ECL emission. The metalized TCPP overcomes the disadvantages of poor stability and easy agglomeration, while the improved hybrid is able to enrich more coreactants and shorten the electron transfer distance. Importantly, this strategy is to anchor the TCPP in an ordered structure through coordination binding, thereby extending the distance between molecules and further eliminating the ACQ effect of the TCPP itself. The optical properties of the obtained LPH are significantly improved. On this basis, using LPH as an efficient transmitter, we constructed an ECL immunosensor for the diagnosis of non-small-cell lung cancer (NSCLC). Significantly, the study not only proposes a new strategy for the diagnosis of NSCLC but also enriches the research of ECL emitters with the ACQ effect."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Natural Science Foundation of Chongqing, China [CSTB2022NSCQ-BHX0006]; Special Funding for Postdoctoral Research Project of Chongqing, China [2021XM2045]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019); Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (82072378, 81601856), Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328), Ba Yu Scholar Program, China (YS2019020), Natural Science Foundation of Chongqing, China ( CSTB2022NSCQ-BHX0006), Special Funding for Postdoctoral Research Project of Chongqing, China (2021XM2045), Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019), Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067), and Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04)."