Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints

作者全名："Yang, Lin; Li, Yan; Hou, Yuanfang; Wu, Yongfu; Tan, Lihong; Mu, Zhenqiang; Zhu, Zhaojing; He, Dan"

作者地址："[Yang, Lin; Li, Yan; Hou, Yuanfang; Tan, Lihong; Mu, Zhenqiang; Zhu, Zhaojing] Chongqing Med & Pharmaceut Coll, Chongqing Pharmaceut Preparat Engn Technol Res Ctr, Chongqing 401331, Peoples R China; [Wu, Yongfu] Tradit Chinese Med Factory Co Ltd, Taiji Grp Chongqing, Chongqing 402284, Peoples R China; [He, Dan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China"

通信作者："He, D (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001070683500001

JCR分区：Q3

影响因子：2.3

年份：2023

卷号：2023

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："An effective and comprehensive quality evaluation method for Liuwei Dihuang pills (LDP) was established by the simultaneous determination of 8 active components in LDP by the quantitative analysis of multicomponents by single marker (QAMS) method and high-performance liquid chromatography (HPLC) fingerprint combined with chemometrics. These 8 active components were determined by QAMS and the external standard method (ESM), and the quantitative results of the two methods were compared to validate the accuracy and feasibility of the QAMS method. 8 active components showed good linear relationships within their ranges, whose average recoveries were 99.7 similar to 102.3%. No significant difference was found (P>0.05) in the quantitative results determined by QAMS and ESM. Furthermore, the fingerprint of LDP was also established, with 11 common peaks identified, and the similarity of the fingerprints of 21 batches of LDP was greater than 0.95. The 21 batches of LDP were basically divided into 3 groups by hierarchical cluster analysis (HCA) and principal component analysis (PCA), and 3 differential markers were screened out by orthogonal partial least squares discriminant analysis (OPLS-DA). The established QAMS method is accurate, economical, fast, and convenient and can simultaneously determine the content of 8 active components in LDP. HPLC fingerprint combined with chemometric analysis more comprehensively evaluated the quality consistency of different batches of LDP and analyzed the markers that cause quality differences between batches. It can provide a scientific basis and reference of quality consistency evaluation for the manufacturers and drug regulatory departments of the preparation."

基金机构："This research was supported by grants from the Science and Technology Research Project of Chongqing Education Commission (KJQN202202807 and KJQN202102809), General Project of Chongqing Science and Technological Committee (CSTB2022NSCQ-MSX0966), and Chongqi [KJQN202202807, KJQN202102809]; Science and Technology Research Project of Chongqing Education Commission [CSTB2022NSCQ-MSX0966]; General Project of Chongqing Science and Technological Committee [YXY2021SD TR01]; Chongqing Medical University Personnel Support Program"

基金资助正文："This research was supported by grants from the Science and Technology Research Project of Chongqing Education Commission (KJQN202202807 and KJQN202102809), General Project of Chongqing Science and Technological Committee (CSTB2022NSCQ-MSX0966), and Chongqing Medical University Personnel Support Program (YXY2021SD TR01)."